Overview

  • Product name

    Anti-PDE10A antibody [EPR22383]
    See all PDE10A primary antibodies
  • Description

    Rabbit monoclonal [EPR22383] to PDE10A
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Fr, IPmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human PDE10A aa 600 to the C-terminus. The exact sequence is proprietary.
    Database link: Q9Y233

  • Positive control

    • WB: HeLa whole cell lysate; Wild-type HAP1 whole cell lysate; Human, mouse and rat brain lysates; Mouse testis lysate. IHC-Fr: Mouse and rat brain tissues. IP: HeLa whole cell lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR22383
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab227829 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 88 kDa (predicted molecular weight: 88 kDa).
IHC-Fr 1/2000.

Perform heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

IP 1/30.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • Target

    • Function

      Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. Can hydrolyze both cAMP and cGMP, but has higher affinity for cAMP and is more efficient with cAMP as substrate.
    • Tissue specificity

      Abundant in the putamen and caudate nucleus regions of brain and testis, moderately expressed in the thyroid gland, pituitary gland, thalamus and cerebellum.
    • Pathway

      Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.
      Purine metabolism; 3',5'-cyclic GMP degradation; GMP from 3',5'-cyclic GMP: step 1/1.
    • Sequence similarities

      Belongs to the cyclic nucleotide phosphodiesterase family.
      Contains 2 GAF domains.
    • Domain

      The tandem GAF domains bind cAMP, and regulate enzyme activity. The binding of cAMP stimulates enzyme activity.
      Composed of a C-terminal catalytic domain containing two divalent metal sites and an N-terminal regulatory domain which contains one cyclic nucleotide-binding region.
    • Cellular localization

      Cytoplasm. Located mostly to soluble cellular fractions.
    • Information by UniProt
    • Database links

    • Alternative names

      • 5''-cyclic phosphodiesterase 10A antibody
      • cAMP and cAMP inhibited cGMP 3'5' cyclic phosphodiesterase 10A antibody
      • cAMP and cAMP-inhibited cGMP 3'' antibody
      • FLJ11894 antibody
      • HSPDE 10A antibody
      • HSPDE10A antibody
      • PDE 10A antibody
      • PDE10_HUMAN antibody
      • Pde10a antibody
      • Phosphodiesterase 10A antibody
      • Phosphodiesterase 10A1 antibody
      see all

    Images

    • All lanes : Anti-PDE10A antibody [EPR22383] (ab227829) at 1/1000 dilution

      Lane 1 : Wild-type HAP1 whole cell lysate
      Lane 2 : PDE10A knockout HAP1 whole cell lysate
      Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 88 kDa
      Observed band size: 88 kDa


      Exposure time: 3 minutes


      Blocking/Dilution buffer: 5% NFDM/TBST.

      ab227829 was shown to specifically react with PDE10A in wild-type HAP1 cells as signal was lost in PDE10A knockout cells. Wild-type and PDE10A knockout samples were subjected to SDS-PAGE. ab227829 and ab181602 (Rabbit anti-GAPDH loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/200,000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/100,000 dilution for 1 hour at room temperature before imaging.

      The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument using the ECL technique.

    • Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse brain tissue labeling PDE10A with ab227829 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Cytoplasmic staining on corpus striatum in mouse brain (PMID:16864896) is observed. Counterstained with DAPI (blue).

      Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    • Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse brain tissue labeling PDE10A with ab227829 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Cytoplasmic staining on mouse caudate-putamen and globus pallidus in mouse brain (PMID:16864896; PMID:28115486) is observed. Counterstained with DAPI (blue).

      Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    • Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat brain tissue labeling PDE10A with ab227829 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Cytoplasmic staining on corpus striatum in rat brain (PMID:16864896) is observed. Counterstained with DAPI (blue).

      Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    • All lanes : Anti-PDE10A antibody [EPR22383] (ab227829) at 1/1000 dilution

      Lane 1 : Human brain lysate
      Lane 2 : Mouse brain lysate
      Lane 3 : Mouse testis lysate
      Lane 4 : Rat brain lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 88 kDa
      Observed band size: 88 kDa



      Exposure time : Lane 1: 3 minutes; Lane 2-3: 3 seconds; Lane 4: 26 seconds.

      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat kidney tissue labeling PDE10A with ab227829 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

      Negative control: No staining on rat kidney (PMID:16864896). Counterstained with DAPI (blue).

      Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    • Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse kidney tissue labeling PDE10A with ab227829 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

      Negative control: No staining on mouse kidney (PMID:16864896). Counterstained with DAPI (blue).

      Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    • PDE10A was immunoprecipitated from 0.35 mg HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab227829 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab227829 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
      Lane 1: HeLa whole cell lysate 10 µg (Input).
      Lane 2: ab227829 IP in HeLa whole cell lysate.
      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab227829 in HeLa whole cell lysate.
      Blocking/Dilution buffer: 5% NFDM/TBST.
      Exposure time: 3 minutes.

    References

    ab227829 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab227829.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up