Anti-PDE10A antibody [OTI3G9] (ab114025)

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Immunocytochemistry/ Immunofluorescence - Anti-PDE10A antibody [OTI3G9] (ab114025)
  • Flow Cytometry - Anti-PDE10A antibody [OTI3G9] (ab114025)

Key features and details

  • Mouse monoclonal [OTI3G9] to PDE10A
  • Suitable for: Flow Cyt, ICC/IF
  • Reacts with: Human, African green monkey
  • Isotype: IgG2b

Overview

  • Product name

    Anti-PDE10A antibody [OTI3G9]
    See all PDE10A primary antibodies

  • Description

    Mouse monoclonal [OTI3G9] to PDE10A

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    African green monkey
    See all applications and species data

  • Immunogen

    Recombinant full length protein corresponding to Human PDE10A aa 1-779. Produced in HEK-293T cells (NP_006652).
    Database link: Q9Y233

  • Positive control

    • ICC/IF: COS-7 cells transiently transfected by pCMV6-ENTRY PDE10A cDNA. Flow Cyt: HEK-293T cells transfected with PDE10A overexpress plasmid.

  • General notes

    Clone OTI3G9 (formerly 3G9).

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 1% BSA, 50% Glycerol
  • Concentration information loading...

  • Purity

    Affinity purified

  • Purification notes

    Purified from cell culture supernatant by affinity chromatography.

  • Clonality

    Monoclonal

  • Clone number

    OTI3G9

  • Isotype

    IgG2b

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab114025 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
ICC/IF
African green monkey
Application Abreviews Notes
Flow Cyt
1/100.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

 
ICC/IF
1/100.
Notes
Flow Cyt
1/100.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

 
ICC/IF
1/100.

Target

  • Function

    Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. Can hydrolyze both cAMP and cGMP, but has higher affinity for cAMP and is more efficient with cAMP as substrate.

  • Tissue specificity

    Abundant in the putamen and caudate nucleus regions of brain and testis, moderately expressed in the thyroid gland, pituitary gland, thalamus and cerebellum.

  • Pathway

    Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.
    Purine metabolism; 3',5'-cyclic GMP degradation; GMP from 3',5'-cyclic GMP: step 1/1.

  • Sequence similarities

    Belongs to the cyclic nucleotide phosphodiesterase family.
    Contains 2 GAF domains.

  • Domain

    The tandem GAF domains bind cAMP, and regulate enzyme activity. The binding of cAMP stimulates enzyme activity.
    Composed of a C-terminal catalytic domain containing two divalent metal sites and an N-terminal regulatory domain which contains one cyclic nucleotide-binding region.

  • Cellular localization

    Cytoplasm. Located mostly to soluble cellular fractions.
  • Information by UniProt

  • Database links

    • Alternative names

      • 5''-cyclic phosphodiesterase 10A antibody
      • cAMP and cAMP inhibited cGMP 3'5' cyclic phosphodiesterase 10A antibody
      • cAMP and cAMP-inhibited cGMP 3'' antibody
      • FLJ11894 antibody
      • HSPDE 10A antibody
      • HSPDE10A antibody
      • PDE 10A antibody
      • PDE10_HUMAN antibody
      • Pde10a antibody
      • Phosphodiesterase 10A antibody
      • Phosphodiesterase 10A1 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-PDE10A antibody [OTI3G9] (ab114025)
      Immunocytochemistry/ Immunofluorescence - Anti-PDE10A antibody [OTI3G9] (ab114025)

      pCMV6-ENTRY PDE10A cDNA transfected COS-7 (African green monkey kidney fibroblast-like cell line) cells stained for PDE10A using ab114025 at a 1/100 dilution in ICC/IF.

    • Flow Cytometry - Anti-PDE10A antibody [OTI3G9] (ab114025)
      Flow Cytometry - Anti-PDE10A antibody [OTI3G9] (ab114025)

      HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with either pCMV6-ENTRY PDE10A (Red) or empty vector control plasmid (Blue) were immunostained with ab114025 at 1/100 and then analyzed by flow cytometry.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab114025? Please let us know so that we can cite the reference in this datasheet.

    ab114025 has not yet been referenced specifically in any publications.

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