Anti-PDE10A antibody [OTI3G9] (ab114025)
Key features and details
- Mouse monoclonal [OTI3G9] to PDE10A
- Suitable for: Flow Cyt (Intra), ICC/IF
- Reacts with: Human, African green monkey
- Isotype: IgG2b
Overview
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Product name
Anti-PDE10A antibody [OTI3G9]
See all PDE10A primary antibodies -
Description
Mouse monoclonal [OTI3G9] to PDE10A -
Host species
Mouse -
Tested applications
Suitable for: Flow Cyt (Intra), ICC/IFmore details -
Species reactivity
Reacts with: Human, African green monkey -
Immunogen
Recombinant full length protein corresponding to Human PDE10A aa 1-779. Produced in HEK-293T cells (NP_006652).
Database link: Q9Y233 -
Positive control
- ICC/IF: COS-7 cells transiently transfected by pCMV6-ENTRY PDE10A cDNA. Flow Cyt (Intra): HEK-293T cells transfected with PDE10A overexpress plasmid.
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General notes
Clone OTI3G9 (formerly 3G9).
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol -
Concentration information loading...
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Purity
Affinity purified -
Purification notes
Purified from cell culture supernatant by affinity chromatography. -
Clonality
Monoclonal -
Clone number
OTI3G9 -
Isotype
IgG2b -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab114025 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/100.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
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ICC/IF |
1/100.
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Notes |
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Flow Cyt (Intra)
1/100. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
ICC/IF
1/100. |
Target
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Function
Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. Can hydrolyze both cAMP and cGMP, but has higher affinity for cAMP and is more efficient with cAMP as substrate. -
Tissue specificity
Abundant in the putamen and caudate nucleus regions of brain and testis, moderately expressed in the thyroid gland, pituitary gland, thalamus and cerebellum. -
Pathway
Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.
Purine metabolism; 3',5'-cyclic GMP degradation; GMP from 3',5'-cyclic GMP: step 1/1. -
Sequence similarities
Belongs to the cyclic nucleotide phosphodiesterase family.
Contains 2 GAF domains. -
Domain
The tandem GAF domains bind cAMP, and regulate enzyme activity. The binding of cAMP stimulates enzyme activity.
Composed of a C-terminal catalytic domain containing two divalent metal sites and an N-terminal regulatory domain which contains one cyclic nucleotide-binding region. -
Cellular localization
Cytoplasm. Located mostly to soluble cellular fractions. - Information by UniProt
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Database links
- Entrez Gene: 10846 Human
- Omim: 610652 Human
- SwissProt: Q9Y233 Human
- Unigene: 348762 Human
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Alternative names
- 5''-cyclic phosphodiesterase 10A antibody
- cAMP and cAMP inhibited cGMP 3'5' cyclic phosphodiesterase 10A antibody
- cAMP and cAMP-inhibited cGMP 3'' antibody
see all
Images
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pCMV6-ENTRY PDE10A cDNA transfected COS-7 (African green monkey kidney fibroblast-like cell line) cells stained for PDE10A using ab114025 at a 1/100 dilution in ICC/IF.
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HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with either pCMV6-ENTRY PDE10A (Red) or empty vector control plasmid (Blue) were immunostained with ab114025 at 1/100 and then analyzed by flow cytometry.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab114025 has not yet been referenced specifically in any publications.