Overview

  • Product name

    Anti-PDE1B antibody [EPR12293(2)]
    See all PDE1B primary antibodies
  • Description

    Rabbit monoclonal [EPR12293(2)] to PDE1B
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human PDE1B aa 1-150. The exact sequence is proprietary.
    Database link: Q01064

  • Positive control

    • WB: Human cerebellum, Human brain, Mouse brain, and Rat brain lysates. IHC-P: human cerebellum, mouse cerebrum, and rat cerebrum tissues.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR12293(2)
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab182565 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).
IHC-P 1/100 - 1/2000.

1:100 in human tissues / 1:2000 in mouse and rat tissues

Target

  • Function

    Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. Has a preference for cGMP as a substrate.
  • Sequence similarities

    Belongs to the cyclic nucleotide phosphodiesterase family. PDE1 subfamily.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • 5''-cyclic nucleotide phosphodiesterase 1B antibody
    • 63 kDa Cam PDE antibody
    • 63 kDa Cam-PDE antibody
    • Calcium/calmodulin dependent 3' 5' cyclic nucleotide Phosphodiesterase 1B antibody
    • Calcium/calmodulin stimulated cyclic nucleotide phosphodiesterase antibody
    • Calcium/calmodulin-dependent 3'' antibody
    • Calmodulin stimulated phosphodiesterase PDE1B1 antibody
    • Cam PDE 1B antibody
    • Cam PDE1B antibody
    • Cam-PDE 1B antibody
    • PDE 1B antibody
    • PDE1B antibody
    • PDE1B_HUMAN antibody
    • PDE1B1 antibody
    • PDES 1B antibody
    • PDES1B antibody
    • Phosphodiesterase 1B antibody
    • Phosphodiesterase 1B calmodulin dependent antibody
    • Presumed 63kDa form of the type 1 cyclic nucleotide phosphodiesterase family known as PDE1B antibody
    see all

Images

  • All lanes : Anti-PDE1B antibody [EPR12293(2)] (ab182565) at 1/1000 dilution (Purified)

    Lane 1 : Human cerebellum lysates
    Lane 2 : Human brain lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 61 kDa
    Observed band size: 61 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat cerebrum tissue sections labeling PDE1B with purified ab182565 at 1/2000 dilution (0.47 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 1 (pH 6.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Anti-PDE1B antibody [EPR12293(2)] (ab182565) at 1/10000 dilution (Purified) + Rat brain lysates at 15 µg

    Secondary
    Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/5000 dilution

    Predicted band size: 61 kDa
    Observed band size: 61 kDa

  • Anti-PDE1B antibody [EPR12293(2)] (ab182565) at 1/5000 dilution (Purified) + Mouse brain lysates at 20 µg

    Secondary
    Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/5000 dilution

    Predicted band size: 61 kDa
    Observed band size: 61 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cerebrum tissue sections labeling PDE1B with purified ab182565 at 1/2000 dilution (0.47 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 1 (pH 6.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebellum tissue sections labeling PDE1B with purified ab182565 at 1/100 dilution (9.43 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 1 (pH 6.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Immunohistochemical analysis of paraffin-embedded Human cerebellum tissue labeling PDE1B with unpurified ab182565 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

References

ab182565 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Striatal Neuron)
Permeabilization
Yes - 0.1% Triton X-100
Specification
Striatal Neuron
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde

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Verified customer

Submitted Apr 13 2017

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