Overview

  • Product name
  • Description
    Rabbit polyclonal to PDE1C
  • Host species
    Rabbit
  • Specificity
    The antibody does not cross react with PDE1A, PDE1B or other PDEs.
  • Tested applications
    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (N terminal).

Properties

Applications

Our Abpromise guarantee covers the use of ab14602 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/250 - 1/500. Predicted molecular weight: 70 kDa. This antibody labels multiple bands in the 70-87 kDa range all believed to be various PDE1C variants.
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. PubMed: 19924104

Target

  • Function
    Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. Has a high affinity for both cAMP and cGMP.
  • Tissue specificity
    Expressed in several tissues, including brain and heart.
  • Sequence similarities
    Belongs to the cyclic nucleotide phosphodiesterase family. PDE1 subfamily.
  • Information by UniProt
  • Database links
  • Alternative names
    • 5''-cyclic nucleotide phosphodiesterase 1C antibody
    • Calcium/calmodulin dependent 3'5' cyclic nucleotide phosphodiesterase 1C antibody
    • Calcium/calmodulin-dependent 3'' antibody
    • Cam PDE 1C antibody
    • Cam-PDE 1C antibody
    • hCam 3 antibody
    • hCam-3 antibody
    • HCAM3 antibody
    • HSPDE1C1A antibody
    • Human 3'5' cyclic nucleotide Phosphodiesterase antibody
    • PDE 1C antibody
    • Pde1c antibody
    • PDE1C_HUMAN antibody
    • Phosphodiesterase 1C antibody
    • Phosphodiesterase 1C calmodulin dependent antibody
    • Phosphodiesterase 1C calmodulin dependent 70kDa antibody
    see all

Images

  • All lanes : Anti-PDE1C antibody (ab14602) at 1/500 dilution

    Lane 1 : Brain lysate
    Lane 2 : Heart lysate
    Lane 3 : Lung lysate
    Lane 4 : Testis lysate
    Lane 5 : Kidney lysate

    Predicted band size: 70 kDa



    Western Blot analysis of PDE1C with ab14602 (1/500). 1- Brain; 2 -Heart; 3 - Lung; 4 - Testis; 5 - Kidney.  

References

This product has been referenced in:
  • He J  et al. Postnatal experience modulates functional properties of mouse olfactory sensory neurons. Eur J Neurosci 36:2452-60 (2012). Read more (PubMed: 22703547) »
  • Giachini FR  et al. Decreased cGMP level contributes to increased contraction in arteries from hypertensive rats: role of phosphodiesterase 1. Hypertension 57:655-63 (2011). WB ; Rat . Read more (PubMed: 21282562) »
See all 4 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Question

DESCRIPTION OF THE PROBLEM No signal or weak signal SAMPLE Mouse cerebellum whole extract PRIMARY ANTIBODY anti-PDE1C (ab14602), diluted in 5% nonfat milk in TBST at 1:250. Incubated over night at 4C with agitation, followed by 4 times wash in TBST (10 min. ea.) DETECTION METHOD When West pico substrate from Pierce was used, no band or background signal was observed. When West femto substrate from Pierce was used, faint multiple bands were observed, however those bands did not seem to be with right size for PDE1C (maybe some of them could be the real bands, but no specifically strong bands were seen). POSITIVE AND NEGATIVE CONTROLS USED No real positive controls were used. When whole brain extract was run instead of cerebellar extract, the same pattern was seen. The cerebellum is the part of the brain which expresses the highest level of PDE1C protein according to multiple reports published so far. ANTIBODY STORAGE CONDITIONS Stored at -80C as aliquotes and brougt back to room temperature just before use (no freeze-thaw cycle). SAMPLE PREPARATION 10mM Tris/HCl pH7.6, 0.33M sucrose, 1mM EDTA, 1mM sodium orthovanadate, 50mM NaF, 25mM pyrophosphate, 100nM calyculin A, 1x phosphatase inhibitor cocktail I (sigma), 1x protease inhibitor cocktail (sigma) Cerebellum was homogenized in 9 volumes of above buffer and adjusted to 1x SDS-PAGE sample buffer by the addition of 5x sample buffer. AMOUNT OF PROTEIN LOADED 20 to 30 micrograms per lane ELECTROPHORESIS/GEL CONDITIONS Reducing SDS-PAGE, 5-20% Gradient SDS-PAGE gel. TRANSFER AND BLOCKING CONDITIONS Transfered onto pre-wet PVDF membrane using typical transfer buffer. Prestained MW marker was transfered nicely. After transfer, the membrane was blocked with 5% nonfat milk diluted in TBST for 1h at room temperature. SECONDARY ANTIBODY goat anti-rabbitIg-HRP conjugate from DAKO, diluted at 1:2000 in 5% nonfat milk in TBST, incubated for 2h at room temperature, followed by 4 times wash in TBST (10 min. ea.) HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 4 HAVE YOU RUN A "NO PRIMARY" CONTROL? No DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes WHAT STEPS HAVE YOU ALTERED? Prepared fresh samples every time. Tried 1:250, 1:500 and 1:1000 dilution of the primary antibody. Used more sensitive substrate (West femto) as mentioned above. Tried whole brain extract in addition to cerebellum samples. The membrane after probed with this antibody (and did not give a band) was re-probed with a different antibody (anti-PKC gamma,it worked beautifully). ADDITIONAL NOTES I have used several different antibodies from Abcam and all of them have worked greatly except for this one. I wish your team can give me a nice technical help to solve the problem with this one. Thanks in advance. Atsushi

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Answer

Thank you for your enquiry. I am sorry to hear you are having a problem with ab14602. I would like to suggest the following modifications to your protocol: Perhaps playing with the milk concentration in the primary antibody solution may help. Removing milk completely may give a lot of background, but if you decrease it to maybe 3%, you may get a better signal. Also, try using 3%BSA in PBS for the blocking step. I would also try running a no primary control definitely because that would tell you if the signal is just from the secondary non specific binding. There is an image on the data sheet and references listed that may help as well. If these tips fail to help you and you have purchased the antibody within the past 90 days, I would be happy to offer you a replacement or refund. Please let me know if this helps and do not hesitate to contact us for further advice.

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Answer

Thank you for your enquiry. I have added a very useful paper to the datasheet for this antibody which will describe the different PEDC1 splice variants in mouse, and their tissue specificity. Yan C et al. The calmodulin-dependent phosphodiesterase gene PDE1C encodes several functionally different splice variants in a tissue-specific manner. J Biol Chem 271:25699-706 (1996). I have included a link to the datasheet at the bottom of this email, which will in turn give you the link to the paper. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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