Anti-PDE2A antibody (ab224616)
Key features and details
- Rabbit polyclonal to PDE2A
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PDE2A antibody
See all PDE2A primary antibodies -
Description
Rabbit polyclonal to PDE2A -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human PDE2A aa 15-226.
Database link: O00408 -
Positive control
- WB: HeLa cell lysate. IHC-P: Human kidney and adrenal gland tissues. ICC/IF: HepG2 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab224616 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/5000. Detects a band of approximately 106 kDa (predicted molecular weight: 106 kDa).
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IHC-P |
1/20 - 1/200.
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ICC/IF |
1/50 - 1/200.
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Notes |
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WB
1/500 - 1/5000. Detects a band of approximately 106 kDa (predicted molecular weight: 106 kDa). |
IHC-P
1/20 - 1/200. |
ICC/IF
1/50 - 1/200. |
Target
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Function
Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. -
Tissue specificity
Expressed in brain and to a lesser extent in heart, placenta, lung, skeletal muscle, kidney and pancreas. -
Sequence similarities
Belongs to the cyclic nucleotide phosphodiesterase family. PDE2 subfamily.
Contains 2 GAF domains. -
Domain
GAF 1 functions as a dimerization domain, whereas GAF 2 binds cGMP, which causes activation of the catalytic activity of the enzyme. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 5138 Human
- Omim: 602658 Human
- SwissProt: O00408 Human
- Unigene: 503163 Human
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Alternative names
- 5''-cyclic phosphodiesterase antibody
- cGMP dependent 3'5' cyclic phosphodiesterase antibody
- cGMP stimulated phosphodiesterase 1 antibody
see all
Images
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Anti-PDE2A antibody (ab224616) at 1/500 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 106 kDa
Observed band size: 106 kDa -
Paraffin-embedded human kidney tissue stained for PDE2A using ab224616 at 1/100 dilution in immunohistochemical analysis.
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Paraffin-embedded human adrenal gland tissue stained for PDE2A using ab224616 at 1/100 dilution in immunohistochemical analysis.
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HepG2 (human liver hepatocellular carcinoma cell line) cells stained for PDE2A (green) using ab224616 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab224616 has been referenced in 1 publication.
- Li SZ et al. miR-139/PDE2A-Notch1 feedback circuit represses stemness of gliomas by inhibiting Wnt/β-catenin signaling. Int J Biol Sci 17:3508-3521 (2021). PubMed: 34512162