Overview

  • Product name

  • Description

    Rabbit polyclonal to PDE2A
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human PDE2A aa 15-226.
    Database link: O00408

  • Positive control

    • WB: HeLa cell lysate. IHC-P: Human kidney and adrenal gland tissues. ICC/IF: HepG2 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab224616 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/5000. Detects a band of approximately 106 kDa (predicted molecular weight: 106 kDa).
IHC-P 1/20 - 1/200.
ICC/IF 1/50 - 1/200.

Target

  • Function

    Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes.
  • Tissue specificity

    Expressed in brain and to a lesser extent in heart, placenta, lung, skeletal muscle, kidney and pancreas.
  • Sequence similarities

    Belongs to the cyclic nucleotide phosphodiesterase family. PDE2 subfamily.
    Contains 2 GAF domains.
  • Domain

    GAF 1 functions as a dimerization domain, whereas GAF 2 binds cGMP, which causes activation of the catalytic activity of the enzyme.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • 5''-cyclic phosphodiesterase antibody
    • cGMP dependent 3'5' cyclic phosphodiesterase antibody
    • cGMP stimulated phosphodiesterase 1 antibody
    • cGMP stimulated phosphodiesterase 4 antibody
    • cGMP-dependent 3'' antibody
    • cGMP-stimulated phosphodiesterase 2 antibody
    • CGS PDE antibody
    • CGS-PDE antibody
    • cGSPDE antibody
    • Cyclic GMP stimulated phosphodiesterase antibody
    • Cyclic GMP-stimulated phosphodiesterase antibody
    • Human cGMP stimulated 3'5' cyclic nucleotide phosphodiesterase antibody
    • PDE 2A antibody
    • PDE 2A1 antibody
    • PDE 2A4 antibody
    • PDE2A antibody
    • PDE2A_HUMAN antibody
    • PDE2A1 antibody
    • PDE2A4 antibody
    • phosphodiesterase 2A antibody
    • Phosphodiesterase 2A cGMP stimulated antibody
    see all

Images

  • Anti-PDE2A antibody (ab224616) at 1/500 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate

    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution

    Predicted band size: 106 kDa
    Observed band size: 106 kDa

  • Paraffin-embedded human kidney tissue stained for PDE2A using ab224616 at 1/100 dilution in immunohistochemical analysis.

  • Paraffin-embedded human adrenal gland tissue stained for PDE2A using ab224616 at 1/100 dilution in immunohistochemical analysis.

  • HepG2 (human liver hepatocellular carcinoma cell line) cells stained for PDE2A (green) using ab224616 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).

References

ab224616 has not yet been referenced specifically in any publications.

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