Anti-PDE3B antibody (ab99289)

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Overview

  • Product name

    Anti-PDE3B antibody
    See all PDE3B primary antibodies

  • Description

    Rabbit polyclonal to PDE3B

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IPmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Elephant

  • Immunogen

    Synthetic peptide, corresponding to a region within the internal sequence amino acids 500-550 of Human PDE3B (NP_000913.2).

  • Positive control

    • Hela whole cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab99289 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 124 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • Function

    Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. May play a role in fat metabolism.

  • Tissue specificity

    Abundant in adipose tissues.

  • Sequence similarities

    Belongs to the cyclic nucleotide phosphodiesterase family. PDE3 subfamily.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • 5''-cyclic phosphodiesterase B antibody
      • Adipocyte cAMP phosphodiesterase cGMP inhibited antibody
      • AI847709 antibody
      • cAMP Phosphodiesterase, adipocyte, cGMP-inhibited antibody
      • CGI-PDE B antibody
      • CGIP1 antibody
      • CGIPDE B antibody
      • CGIPDE1 antibody
      • cGMP inhibited 3 5 cyclic phosphodiesterase B antibody
      • CGMP inhibited cAMP phophodiesterase antibody
      • cGMP-inhibited 3'' antibody
      • Cyclic GMP inhibited phosphodiesterase B antibody
      • Cyclic GMP-inhibited phosphodiesterase B antibody
      • Cyclic nucleotide phosphodiesterase antibody
      • HcGIP1 antibody
      • HcGIP1, rat, homolog of antibody
      • Pde3b antibody
      • PDE3B_HUMAN antibody
      • Phosphodiesterase 3B antibody
      • Phosphodiesterase 3B cGMP inhibited antibody
      see all

    Images

    • Western blot - Anti-PDE3B antibody (ab99289)
      Western blot - Anti-PDE3B antibody (ab99289)
      All lanes : Anti-PDE3B antibody (ab99289) at 0.1 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg

      Developed using the ECL technique.

      Predicted band size: 124 kDa


      Exposure time: 3 minutes
    • Immunoprecipitation - Anti-PDE3B antibody (ab99289)
      Immunoprecipitation - Anti-PDE3B antibody (ab99289)
      Lane 1: Detection of Human PDE3B by Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using ab99289 at 3 µg/mg lysate for IP.
      Lane 2: Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using Control IgG.
      Subsequent Western blot detection used ab99289 at 1 µg/ml.
      Developed using the ECL technique with an exposure time of 30 seconds.

    References

    ab99289 has not yet been referenced specifically in any publications.

    Publishing research using ab99289? Please let us know so that we can cite the reference in this datasheet.

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