Overview

  • Product name
    Anti-PDE3B antibody
    See all PDE3B primary antibodies
  • Description
    Rabbit polyclonal to PDE3B
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Elephant
  • Immunogen

    Synthetic peptide, corresponding to a region within the internal sequence amino acids 500-550 of Human PDE3B (NP_000913.2).

  • Positive control
    • Hela whole cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab99289 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 124 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • Function
    Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. May play a role in fat metabolism.
  • Tissue specificity
    Abundant in adipose tissues.
  • Sequence similarities
    Belongs to the cyclic nucleotide phosphodiesterase family. PDE3 subfamily.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
    • Alternative names
      • 5''-cyclic phosphodiesterase B antibody
      • Adipocyte cAMP phosphodiesterase cGMP inhibited antibody
      • AI847709 antibody
      • cAMP Phosphodiesterase, adipocyte, cGMP-inhibited antibody
      • CGI-PDE B antibody
      • CGIP1 antibody
      • CGIPDE B antibody
      • CGIPDE1 antibody
      • cGMP inhibited 3 5 cyclic phosphodiesterase B antibody
      • CGMP inhibited cAMP phophodiesterase antibody
      • cGMP-inhibited 3'' antibody
      • Cyclic GMP inhibited phosphodiesterase B antibody
      • Cyclic GMP-inhibited phosphodiesterase B antibody
      • Cyclic nucleotide phosphodiesterase antibody
      • HcGIP1 antibody
      • HcGIP1, rat, homolog of antibody
      • Pde3b antibody
      • PDE3B_HUMAN antibody
      • Phosphodiesterase 3B antibody
      • Phosphodiesterase 3B cGMP inhibited antibody
      see all

    Images

    • Western blot - Anti-PDE3B antibody (ab99289)
      Western blot - Anti-PDE3B antibody (ab99289)
      All lanes : Anti-PDE3B antibody (ab99289) at 0.1 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg

      Developed using the ECL technique.

      Predicted band size: 124 kDa


      Exposure time: 3 minutes
    • Immunoprecipitation - Anti-PDE3B antibody (ab99289)
      Immunoprecipitation - Anti-PDE3B antibody (ab99289)
      Lane 1: Detection of Human PDE3B by Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using ab99289 at 3 µg/mg lysate for IP.
      Lane 2: Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using Control IgG.
      Subsequent Western blot detection used ab99289 at 1 µg/ml.
      Developed using the ECL technique with an exposure time of 30 seconds.

    References

    ab99289 has not yet been referenced specifically in any publications.

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