Anti-PDE3B antibody (ab99289)
Key features and details
- Rabbit polyclonal to PDE3B
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PDE3B antibody
See all PDE3B primary antibodies -
Description
Rabbit polyclonal to PDE3B -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Elephant
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Immunogen
Synthetic peptide, corresponding to a region within the internal sequence amino acids 500-550 of Human PDE3B (NP_000913.2).
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Positive control
- Hela whole cell lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab99289 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 124 kDa.
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| IP |
Use at 2-5 µg/mg of lysate.
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| Notes |
|---|
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WB
1/2000 - 1/10000. Predicted molecular weight: 124 kDa. |
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IP
Use at 2-5 µg/mg of lysate. |
Target
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Function
Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. May play a role in fat metabolism. -
Tissue specificity
Abundant in adipose tissues. -
Sequence similarities
Belongs to the cyclic nucleotide phosphodiesterase family. PDE3 subfamily. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 5140 Human
- Omim: 602047 Human
- SwissProt: Q13370 Human
- Unigene: 445711 Human
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Alternative names
- 5''-cyclic phosphodiesterase B antibody
- Adipocyte cAMP phosphodiesterase cGMP inhibited antibody
- AI847709 antibody
see all
Images
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Western blot - Anti-PDE3B antibody (ab99289)All lanes : Anti-PDE3B antibody (ab99289) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 124 kDa
Exposure time: 3 minutes -
Immunoprecipitation - Anti-PDE3B antibody (ab99289)Lane 1: Detection of Human PDE3B by Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using ab99289 at 3 µg/mg lysate for IP.
Lane 2: Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using Control IgG.
Subsequent Western blot detection used ab99289 at 1 µg/ml.
Developed using the ECL technique with an exposure time of 30 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab99289 has not yet been referenced specifically in any publications.