Key features and details
- Rabbit polyclonal to PDE3B
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PDE3B antibody
See all PDE3B primary antibodies
DescriptionRabbit polyclonal to PDE3B
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Horse, Chicken, Turkey, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
Synthetic peptide corresponding to a region between residue 1062 and 1112 of human PDE3B. (NP_000913.2)
- HeLa whole cell lysate (ab150035)
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab99290 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 124 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionCyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. May play a role in fat metabolism.
Tissue specificityAbundant in adipose tissues.
Sequence similaritiesBelongs to the cyclic nucleotide phosphodiesterase family. PDE3 subfamily.
- Information by UniProt
- 5''-cyclic phosphodiesterase B antibody
- Adipocyte cAMP phosphodiesterase cGMP inhibited antibody
- AI847709 antibody
All lanes : Anti-PDE3B antibody (ab99290) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Predicted band size: 124 kDa
Lane 1: Detection of Human PDE3B by Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using an antibody recognising an upstream epitope of PDE3B.
Lane 2: Detection of Human PDE3B by Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using ab99290 at 3 µg/mg lysate for IP.
Lane 3 : Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using Control IgG.
Subsequent Western blot detection used ab99290 at 1 µg/ml. Developed using the ECL technique with an exposure time of 10 seconds.
ab99290 has been referenced in 1 publication.
- Ramshanker N et al. Effects of short-term prednisolone treatment on indices of lipolysis and lipase signaling in abdominal adipose tissue in healthy humans. Metabolism 99:1-10 (2019). PubMed: 31260678