Anti-PDE4 antibody (ab14628)
Key features and details
- Rabbit polyclonal to PDE4
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-PDE4 antibody
See all PDE4 primary antibodies -
Description
Rabbit polyclonal to PDE4 -
Host species
Rabbit -
Specificity
This antibody detects all known PDE4 A and D variants including PDE4A1, A5, Ax, A8 and PDE4D1-D5. It has low affinity for PDE4B variants. -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Rat brain.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Thimerosal (merthiolate) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab14628 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent dilution.
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ICC/IF |
Use a concentration of 1 µg/ml.
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Notes |
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WB
Use at an assay dependent dilution. |
ICC/IF
Use a concentration of 1 µg/ml. |
Target
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Relevance
The cAMP-specific Phosphodiesterase type 4 (PDE4) family is comprised of 4 genes (PDE4 A, B, C and D) with multiple splice variants generated by RNA splicing and use of alternate initiation sites. The cAMP-dependent protein kinase A modulates the enzyme activity of some PDE4 family members by phosphorylation / dephosphorylation mechanisms. Such short-term regulatory mechanisms are necessary for rapid changes in the cAMP levels in cells. PDE4 family selective inhibitor, rolipram inhibits all members of the PDE4 family. In rodents inhibition of PDE4 enzymes by rolipram attenuated short- and long-term memory impairment produced by the administration of scopolamine and MK801. Interaction of these enzymes with cellular scaffolding proteins plays important role in PDE4 compartmentalization and cAMP-dependent signaling. -
Cellular localization
Cell Membrane -
Database links
- Entrez Gene: 5141 Human
- Entrez Gene: 5142 Human
- Entrez Gene: 5143 Human
- Entrez Gene: 5144 Human
- SwissProt: P27815 Human
- SwissProt: Q07343 Human
- SwissProt: Q08493 Human
- SwissProt: Q08499 Human
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Alternative names
- PDE4A antibody
- PDE4B antibody
- PDE4C antibody
see all
Images
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ab 14628 detecting 3 isoforms of PDE4A subclasses (PDE4A1, PDE4A5 and PDE4Ax), two isoforms of PDE4D (PDE4D1, PDE4D3) in rat brain by Western blot.
ab 14628 detecting 3 isoforms of PDE4A subclasses (PDE4A1, PDE4A5 and PDE4Ax) and two isoforms of PDE4D (PDE4D1, PDE4D3) in rat brain by Western blot.
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ICC/IF image of ab14628 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14628, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (17)
ab14628 has been referenced in 17 publications.
- Li S et al. Transcription factor JDP2 activates PDE4B to participate in hypoxia/reoxygenation-induced H9c2 cell injury. Exp Ther Med 23:340 (2022). PubMed: 35401806
- Shrestha J et al. HIV-1 gp120 Impairs Spatial Memory Through Cyclic AMP Response Element-Binding Protein. Front Aging Neurosci 14:811481 (2022). PubMed: 35615594
- Dong XL et al. The protective effect of the PDE-4 inhibitor rolipram on intracerebral haemorrhage is associated with the cAMP/AMPK/SIRT1 pathway. Sci Rep 11:19737 (2021). PubMed: 34611179
- Jayachandran R et al. Disruption of Coronin 1 Signaling in T Cells Promotes Allograft Tolerance while Maintaining Anti-Pathogen Immunity. Immunity 50:152-165.e8 (2019). PubMed: 30611611
- Dey S et al. Cyclic AMP and glycogen synthase kinase 3 form a regulatory loop in spermatozoa. J Cell Physiol 233:7239-7252 (2018). PubMed: 29574946