Overview

  • Product name
    Anti-PDE4 antibody
  • Description
    Rabbit polyclonal to PDE4
  • Host species
    Rabbit
  • Specificity
    This antibody detects all known PDE4 A and D variants including PDE4A1, A5, Ax, A8 and PDE4D1-D5. It has low affinity for PDE4B variants.
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive).

  • Positive control
    • Rat brain.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Thimerosal (merthiolate)
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab14628 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.
ICC/IF Use a concentration of 1 µg/ml.

Target

  • Relevance
    The cAMP-specific Phosphodiesterase type 4 (PDE4) family is comprised of 4 genes (PDE4 A, B, C and D) with multiple splice variants generated by RNA splicing and use of alternate initiation sites. The cAMP-dependent protein kinase A modulates the enzyme activity of some PDE4 family members by phosphorylation / dephosphorylation mechanisms. Such short-term regulatory mechanisms are necessary for rapid changes in the cAMP levels in cells. PDE4 family selective inhibitor, rolipram inhibits all members of the PDE4 family. In rodents inhibition of PDE4 enzymes by rolipram attenuated short- and long-term memory impairment produced by the administration of scopolamine and MK801. Interaction of these enzymes with cellular scaffolding proteins plays important role in PDE4 compartmentalization and cAMP-dependent signaling.
  • Cellular localization
    Cell Membrane
  • Database links
  • Alternative names
    • PDE4A antibody
    • PDE4B antibody
    • PDE4C antibody
    • PDE4D antibody
    • Phosphodiesterase 4 antibody
    • Phosphodiesterase 4A antibody
    • Phosphodiesterase 4B antibody
    • Phosphodiesterase 4C antibody
    • Phosphodiesterase 4D antibody
    see all

Images

  • ab 14628 detecting 3 isoforms of PDE4A subclasses (PDE4A1, PDE4A5 and PDE4Ax), two isoforms of PDE4D (PDE4D1, PDE4D3) in rat brain by Western blot.

    ab 14628 detecting 3 isoforms of PDE4A subclasses (PDE4A1, PDE4A5 and PDE4Ax) and two isoforms of PDE4D (PDE4D1, PDE4D3) in rat brain by Western blot.

  • ICC/IF image of ab14628 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14628, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:
  • Sharma M  et al. East Indian Sandalwood Oil Is a Phosphodiesterase Inhibitor: A New Therapeutic Option in the Treatment of Inflammatory Skin Disease. Front Pharmacol 9:200 (2018). Read more (PubMed: 29593534) »
  • Li Q  et al. Phosphodiesterase-4 inhibition confers a neuroprotective efficacy against early brain injury following experimental subarachnoid hemorrhage in rats by attenuating neuronal apoptosis through the SIRT1/Akt pathway. Biomed Pharmacother 99:947-955 (2018). IHC-Fr ; Rat . Read more (PubMed: 29710495) »
See all 11 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

I have received a reply from the originator of the product and have attached it below. "Yes, this band was initially characterized as PDE4B4 (before Graemme bolger cloned the PDE4B4) as reported in Biology of Reproduction paper, 2001." I hope this reply will be able to answer your question. Should you have any other questions, I will be more than happy to forward them again.

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Answer

Thank you for your patience. I have contacted the originator of the product for the information you require. Please find the molecular weight band information below. Molecular Wt: PDE4A5 110kDa PDE4Ax 102kDa PDE4D1 68 kDa PDE4D3 95 kDa PDE4A1 69kDa PDE4D2 68 kDa PDE4D4 119 kDa PDE4D5 105 kDa These are the MW of different PDE4 isoforms. PDE4B1 107 kDa PDE4B2 78 kDa PDE4B3 100 kDa PDE4B4 66 kDa I hope the above information will be useful. Should you require any other assistance, please do not hesitate to contact me.

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Answer

Thank you for your enquiry. This antibody will label most of the PDE4 proteins from PDE4A (PDE4A1,65 kDa; PDE4A5, 106 kDa, PDE4Ax 102 kDa);in PDE4B (generally it labels only PDE4B3 (100 kDa); PDE4D (PDE4D1, D2, D3, D4, D5) provided these proteins are expresed in your sample. Please contact us again if your require further assistance.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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