• Product name
  • Description
    Rabbit polyclonal to PDE4A
  • Host species
  • Specificity
    This antibody labels all known PDE4A variants including PDE4A1 (66 kDa), A5 (109 kDa), A8 (106 kDa), Ax (102 kDa) and a 76 kDa testis specific A variant.
  • Tested applications
    Suitable for: ICC/IF, WB, IP, ICC, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Human
  • Immunogen

    Synthetic peptide corresponding to the C-terminal region of PDE4A (common to all PDE4A proteins).

  • Positive control
    • recombinant protein (PDE4A1, PDE4A5, PDE4A8, PDE4A10, PDE4A11)


  • Form
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab14607 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB 1/1000. Predicted molecular weight: 98 kDa.Can be blocked with PDE4A peptide (ab169988). Detects bands at 66 kDa (PDE4A1), 109 kDa (PDE4A5), 106 kDa(PDE4A8), 102 kDa (PDE4Ax) and 76 kDa (testis specific PDE4A variant).
IP 1/250.
ICC 1/250.
IHC-P Use at an assay dependent concentration. PubMed: 19924104


  • Function
    Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.
  • Tissue specificity
    Isoform 1 is widely expressed. Isoform 2 is abundant in liver, stomach, testis, thyroid and adrenal glands. It is also found in placenta, kidney, pancreas, ovary, uterus, skin, monocytes, mast cells, macrophages, as well as in bronchial smooth muscle. Isoform 6 is expressed at high levels in the heart and small intestine. It is also found in the brain, kidney, spleen, colon, salivary gland, ovary and peripheral blood lymphocytes. Isoform 7 is expressed predominantly in skeletal muscle and brain and at lower levels in the testis. Isoform 7 is expressed in the brain. Found in specific neuronal subpopulations in cortex, spinal cord and cerebellum (at protein level).
  • Pathway
    Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.
  • Sequence similarities
    Belongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily.
  • Post-translational
    Phosphorylated at Ser-686 and Ser-688 when expressed in S.frugiperda cells. Isoform 2 and isoform 7 are activated by phosphorylation at Ser-119 and Ser-123 respectively by PKA.
    Proteolytically cleaved by caspase-3.
  • Cellular localization
    Cytoplasm > perinuclear region; Cytoplasm > perinuclear region. Cell projection > ruffle membrane; Cytoplasm. Membrane. Predominantly cytoplasmic and Membrane. Isoform 4 has propensity for association with membranes.
  • Information by UniProt
  • Database links
  • Alternative names
    • 5''-cyclic phosphodiesterase 4A antibody
    • cAMP specific phosphodiesterase antibody
    • cAMP-specific 3'' antibody
    • cAMP-specific 3',5'-cyclic phosphodiesterase 4A antibody
    • DPDE2 antibody
    • dunce like phosphodiesterase E2 antibody
    • PDE 4A antibody
    • PDE4 antibody
    • PDE46 antibody
    • PDE4A antibody
    • PDE4A_HUMAN antibody
    • PDE4A11 antibody
    • Phosphodiesterase 4A antibody
    • phosphodiesterase 4A cAMP specific antibody
    • Phosphodiesterase 4A, cAMP-specific (dunce antibody
    • phosphodiesterase E2 dunce homolog antibody
    • Phosphodiesterase E2 dunce homolog, Drosophila antibody
    • phosphodiesterase isozyme 4 antibody
    • phosphodiesterase type 4A11 antibody
    see all


This product has been referenced in:
  • Yang ST  et al. Core Differences in Synaptic Signaling Between Primary Visual and Dorsolateral Prefrontal Cortex. Cereb Cortex 28:1458-1471 (2018). Read more (PubMed: 29351585) »
  • Peng Y  et al. PDE4a predicts poor prognosis and promotes metastasis by inducing epithelial-mesenchymal transition in hepatocellular carcinoma. J Cancer 9:2389-2396 (2018). Read more (PubMed: 30026835) »
See all 18 Publications for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A


The buffer contains mainly Tris/glycine buffer pH 7.4-7.8; cryo-protective agents, stabilizing proteins (BSA) and glycerol. There is also sodium azide as preservative. The exact composition of this buffer is proprietary.

Read More


Thank you for your enquiry. 1) PDE4A antibody (ab14607) does not cross react to any known PDE4 subtype except all the PDE4A subtype variants including PDE4A1, A5, Ax, A8, it has little or no reactivity towards PDE4A10 and PDE4A11. We have tested for cross reactivity against other type of PDE and so far unable to pick up any other protein in the PDE class. This antibody does not label human PDE4 subtypes. 2) PDE4B antibody (ab14612) labels all the PDE4B1, B2, B3, B4 variants (based on cross reactivity towards rec proteins). In tissue we pick up 2 extra bands, which may be phosphorylated proteins as they tend to migrate a little higher. This product has no cross reactivity towards any other PDE4 or PDE protein that we can detect. 3) PDE4C antibody (ab14608) labels only PDE4C variants, we have seen cross reactivity against PDE4C1, C2, C4, there are few other variants that may have been picked up but we are not sure. In our sample, C3 was a minor band. The antibody does not cross react to other PDE4 to PDE family members that we can detect. 4) PDE4D antibody (ab14613) labels all amino terminal end variansts (PDE4D1-D9) with no cross reactivity towards any other isoform based on rec-protein data. This antibody also labels phosphorylated forms of PDE4Ds. I hope this information will be helpful.

Read More


I am sorry these products did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued free of charge replacements for ab14611 and ab14613with the order number 1137676.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Read More


Thank you for contacting us.

I have been in contact with the lab about this product, the epitope that this product recognizes is not mapped, so we don't know if it would recognize all the isoforms of PDE4A.
I would also like to inform you that according to the lab ab123925 was negative on lung carcinoma.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Western blot
Rat Cell lysate - whole cell (Rat fibroblasts)
Loading amount
5 µg
Rat fibroblasts
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 28°C

Abcam user community

Verified customer

Submitted Sep 19 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Immunocytochemistry/ Immunofluorescence
Mouse Cell (vomeronasal organ VNO)
vomeronasal organ VNO
Blocking step
gelatine plus serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5

Abcam user community

Verified customer

Submitted Oct 25 2006


Sign up