Key features and details
- Rabbit polyclonal to PDE4A
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PDE4A antibody
See all PDE4A primary antibodies
DescriptionRabbit polyclonal to PDE4A
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human PDE4A (internal sequence). (NP_001104777).
Database link: P27815
- HeLa and A172 cell lysates; Human thyroid cancer tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol, 49% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab200383 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Predicted molecular weight: 98 kDa.|
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
FunctionHydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.
Tissue specificityIsoform 1 is widely expressed. Isoform 2 is abundant in liver, stomach, testis, thyroid and adrenal glands. It is also found in placenta, kidney, pancreas, ovary, uterus, skin, monocytes, mast cells, macrophages, as well as in bronchial smooth muscle. Isoform 6 is expressed at high levels in the heart and small intestine. It is also found in the brain, kidney, spleen, colon, salivary gland, ovary and peripheral blood lymphocytes. Isoform 7 is expressed predominantly in skeletal muscle and brain and at lower levels in the testis. Isoform 7 is expressed in the brain. Found in specific neuronal subpopulations in cortex, spinal cord and cerebellum (at protein level).
PathwayPurine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.
Sequence similaritiesBelongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily.
modificationsPhosphorylated at Ser-686 and Ser-688 when expressed in S.frugiperda cells. Isoform 2 and isoform 7 are activated by phosphorylation at Ser-119 and Ser-123 respectively by PKA.
Proteolytically cleaved by caspase-3.
Cellular localizationCytoplasm > perinuclear region; Cytoplasm > perinuclear region. Cell projection > ruffle membrane; Cytoplasm. Membrane. Predominantly cytoplasmic and Membrane. Isoform 4 has propensity for association with membranes.
- Information by UniProt
- 5''-cyclic phosphodiesterase 4A antibody
- cAMP specific phosphodiesterase antibody
- cAMP-specific 3'' antibody
All lanes : Anti-PDE4A antibody (ab200383) at 1/300 dilution
Lane 1 : HeLa cell lysate
Lane 2 : A172 cell lysate
Lysates/proteins at 40 µg per lane.
Predicted band size: 98 kDa
Exposure time: 20 seconds
Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue labeling PDE4A with ab200383 at 1/40 dilution.
ab200383 has been referenced in 1 publication.
- Ale-Agha N et al. CDKN1B/p27 is localized in mitochondria and improves respiration-dependent processes in the cardiovascular system-New mode of action for caffeine. PLoS Biol 16:e2004408 (2018). PubMed: 29927970