Anti-PDE4A antibody (ab200383)
Key features and details
- Rabbit polyclonal to PDE4A
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PDE4A antibody
See all PDE4A primary antibodies -
Description
Rabbit polyclonal to PDE4A -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human PDE4A (internal sequence). (NP_001104777).
Database link: P27815 -
Positive control
- HeLa and A172 cell lysates; Human thyroid cancer tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab200383 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/2000. Predicted molecular weight: 98 kDa.
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IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Notes |
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WB
1/500 - 1/2000. Predicted molecular weight: 98 kDa. |
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. -
Tissue specificity
Isoform 1 is widely expressed. Isoform 2 is abundant in liver, stomach, testis, thyroid and adrenal glands. It is also found in placenta, kidney, pancreas, ovary, uterus, skin, monocytes, mast cells, macrophages, as well as in bronchial smooth muscle. Isoform 6 is expressed at high levels in the heart and small intestine. It is also found in the brain, kidney, spleen, colon, salivary gland, ovary and peripheral blood lymphocytes. Isoform 7 is expressed predominantly in skeletal muscle and brain and at lower levels in the testis. Isoform 7 is expressed in the brain. Found in specific neuronal subpopulations in cortex, spinal cord and cerebellum (at protein level). -
Pathway
Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1. -
Sequence similarities
Belongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily. -
Post-translational
modificationsPhosphorylated at Ser-686 and Ser-688 when expressed in S.frugiperda cells. Isoform 2 and isoform 7 are activated by phosphorylation at Ser-119 and Ser-123 respectively by PKA.
Proteolytically cleaved by caspase-3. -
Cellular localization
Cytoplasm > perinuclear region; Cytoplasm > perinuclear region. Cell projection > ruffle membrane; Cytoplasm. Membrane. Predominantly cytoplasmic and Membrane. Isoform 4 has propensity for association with membranes. - Information by UniProt
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Database links
- Entrez Gene: 5141 Human
- Omim: 600126 Human
- SwissProt: P27815 Human
- Unigene: 89901 Human
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Alternative names
- 5''-cyclic phosphodiesterase 4A antibody
- cAMP specific phosphodiesterase antibody
- cAMP-specific 3'' antibody
see all
Images
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All lanes : Anti-PDE4A antibody (ab200383) at 1/300 dilution
Lane 1 : HeLa cell lysate
Lane 2 : A172 cell lysate
Lysates/proteins at 40 µg per lane.
Predicted band size: 98 kDa
Exposure time: 20 seconds6% SDS-PAGE.
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Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue labeling PDE4A with ab200383 at 1/40 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab200383 has been referenced in 3 publications.
- Meng S et al. CircAXL Knockdown Alleviates Aβ1-42-Induced Neurotoxicity in Alzheimer's Disease via Repressing PDE4A by Releasing miR-1306-5p. Neurochem Res 47:1707-1720 (2022). PubMed: 35229272
- Wang J et al. H2 S catalysed by CBS regulates testosterone synthesis through affecting the sulfhydrylation of PDE. J Cell Mol Med 25:3460-3468 (2021). PubMed: 33713531
- Ale-Agha N et al. CDKN1B/p27 is localized in mitochondria and improves respiration-dependent processes in the cardiovascular system-New mode of action for caffeine. PLoS Biol 16:e2004408 (2018). PubMed: 29927970