Key features and details
- Rabbit polyclonal to PDE4A (phospho S)
- Suitable for: WB
- Reacts with: Mouse, Rat, Rabbit, Human
- Isotype: IgG
Product nameAnti-PDE4A (phospho S) antibody
See all PDE4A primary antibodies
DescriptionRabbit polyclonal to PDE4A (phospho S)
SpecificityThis antibody detects all known long-from variants of PDE4A, including PDE4A4, PDE4A5, PDE4A8, PDE4Ax when phosphorylated at the PKA phophorylation site. It will not cross-react with PDE4A phosphorylated at the ERK phosphorylation site. It does not cross-react with PDE4B, PDE4D or any other member of the PDE family.
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Rat, Rabbit, Human
Synthetic peptide corresponding to PKA phosphorylation site on PDE4A long form enzymes. The peptide was synthesized with phosphorylated serine.
This antibody is ideal for studying phosphorylation dependent signal transduction research of PDE4 activation.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThe phospho-specific antibodies were selected by differential affinity chromatography matrices of phospho and non-phospho peptide columns.
Primary antibody notesThis antibody is ideal for studying phosphorylation dependent signal transduction research of PDE4 activation.
Our Abpromise guarantee covers the use of ab14610 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2500. Detects a band of approximately 71 kDa (predicted molecular weight: 66 kDa). Detects bands at 66 kDa (PDE4A1), 109 kDa (PDE4A5), 106 kDa(PDE4A8), 102 kDa (PDE4Ax) and 76 kDa (testis specific PDE4A variant).|
FunctionHydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.
Tissue specificityIsoform 1 is widely expressed. Isoform 2 is abundant in liver, stomach, testis, thyroid and adrenal glands. It is also found in placenta, kidney, pancreas, ovary, uterus, skin, monocytes, mast cells, macrophages, as well as in bronchial smooth muscle. Isoform 6 is expressed at high levels in the heart and small intestine. It is also found in the brain, kidney, spleen, colon, salivary gland, ovary and peripheral blood lymphocytes. Isoform 7 is expressed predominantly in skeletal muscle and brain and at lower levels in the testis. Isoform 7 is expressed in the brain. Found in specific neuronal subpopulations in cortex, spinal cord and cerebellum (at protein level).
PathwayPurine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.
Sequence similaritiesBelongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily.
modificationsPhosphorylated at Ser-686 and Ser-688 when expressed in S.frugiperda cells. Isoform 2 and isoform 7 are activated by phosphorylation at Ser-119 and Ser-123 respectively by PKA.
Proteolytically cleaved by caspase-3.
Cellular localizationCytoplasm > perinuclear region; Cytoplasm > perinuclear region. Cell projection > ruffle membrane; Cytoplasm. Membrane. Predominantly cytoplasmic and Membrane. Isoform 4 has propensity for association with membranes.
- Information by UniProt
- 5''-cyclic phosphodiesterase 4A antibody
- cAMP specific phosphodiesterase antibody
- cAMP-specific 3'' antibody
Anti-PDE4A (phospho S) antibody (ab14610) at 1 µg/ml + Human thyroid normal tissue lysate - total protein (ab29636) at 10 µg
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 66 kDa
Observed band size: 71 kDa why is the actual band size different from the predicted?
Additional bands at: 53 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
The 71 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to PDE4A.
ab14610 has been referenced in 3 publications.
- Hu Z et al. Fidgetin regulates cultured astrocyte migration by severing tyrosinated microtubules at the leading edge. Mol Biol Cell 28:545-553 (2017). WB . PubMed: 27974640
- Schaal SM et al. The Therapeutic Profile of Rolipram, PDE Target and Mechanism of Action as a Neuroprotectant following Spinal Cord Injury. PLoS One 7:e43634 (2012). WB ; Rat . PubMed: 23028463
- Zhu B et al. Activation and induction of cyclic AMP phosphodiesterase (PDE4) in rat pulmonary microvascular endothelial cells. Biochem Pharmacol 68:479-91 (2004). PubMed: 15242814