• Product name

  • Description

    Rabbit polyclonal to PDE4B
  • Host species

  • Specificity

    This antibody detects all PDE4B proteins, including PDE4B1, PDE4B2, PDE4B3 and a 66 kDa PDE4B variant. It does not cross-react with PDE4A, PDE4C or PDE4D.
  • Tested applications

    Suitable for: WB, ICC/IF, IP, ICC, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (N terminal).


  • Form

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituents: PBS, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

  • Isotype

  • Research areas


Our Abpromise guarantee covers the use of ab14611 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1500. Predicted molecular weight: 107 kDa. Detects bands of 107 kDa (PDE4B1), 78 kDa (PDE4B2), 100 kDa (PDE4B3) and 66 kDa (PDE4B4).
ICC/IF Use at an assay dependent concentration.
IP 1/350.
ICC 1/300.
IHC-P Use at an assay dependent concentration. PubMed: 19924104


  • Function

    Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May be involved in mediating central nervous system effects of therapeutic agents ranging from antidepressants to antiasthmatic and anti-inflammatory agents.
  • Tissue specificity

    Expressed in brain, heart, lung and skeletal muscle.
  • Pathway

    Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.
  • Sequence similarities

    Belongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily.
  • Information by UniProt
  • Database links

  • Alternative names

    • 5''-cyclic phosphodiesterase 4B antibody
    • cAMP-specific 3'' antibody
    • cAMP-specific 3',5'-cyclic phosphodiesterase 4B antibody
    • cAMP-specific phosphodiesterase-4 B isoform antibody
    • DKFZp686F2182 antibody
    • DPDE4 antibody
    • dunce-like phosphodiesterase E4 antibody
    • MGC126529 antibody
    • OTTHUMP00000010652 antibody
    • OTTHUMP00000010653 antibody
    • OTTHUMP00000010654 antibody
    • OTTHUMP00000010656 antibody
    • OTTHUMP00000232365 antibody
    • PDE 4B antibody
    • PDE IVB antibody
    • PDE32 antibody
    • Pde4b antibody
    • PDE4B_HUMAN antibody
    • PDE4B5 antibody
    • PDEIVB antibody
    • Phosphodiesterase 4B antibody
    • Phosphodiesterase 4B cAMP specific antibody
    • Phosphodiesterase 4B, cAMP specific (phosphodiesterase E4 dunce homolog, Drosophila) antibody
    see all


  • All lanes : Anti-PDE4B antibody (ab14611) at 1/5000 dilution

    All lanes : Rat mesangial cells - whole cell lysate

    Lysates/proteins at 5 µg per lane.

    All lanes : HRP conjugated goat anti-rabbit at 1/1000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 107 kDa
    Observed band size: 106 kDa
    why is the actual band size different from the predicted?

    Western blot of 3 collected lysates from untreated rat mesangial cells using ab14611.

    See Abreview


This product has been referenced in:

  • Myers SA  et al. Following spinal cord injury, PDE4B drives an acute, local inflammatory response and a chronic, systemic response exacerbated by gut dysbiosis and endotoxemia. Neurobiol Dis 124:353-363 (2018). Read more (PubMed: 30557659) »
  • Cheng H  et al. siRNA-mediated silencing of phosphodiesterase 4B expression affects the production of cytokines in endotoxin-stimulated primary cultured microglia. Exp Ther Med 12:2257-2264 (2016). WB . Read more (PubMed: 27698721) »
See all 9 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A


I am sorry these products did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued free of charge replacements for ab14611 and ab14613with the order number 1137676.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Read More


Attached is our blot for PDE4B1, B2, B3 and B4 proteins, there are several papers where similar analyses were published from different labs.
I am sorry but the immunogen sequences are not available.
I hope this is helpful. Please contact us agin if you have any further questions.

Read More
Western blot
Rat Cell lysate - whole cell (Mesangial Cells)
Loading amount
5 µg
Mesangial Cells
1mM epsilon aminocaproic acid for 24hr
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C

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