Recombinant Anti-PDE4B antibody [EPR11830] (ab170939)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11830] to PDE4B
- Suitable for: WB, Flow Cyt
- Knockout validated
- Reacts with: Mouse, Human
Overview
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Product name
Anti-PDE4B antibody [EPR11830]
See all PDE4B primary antibodies -
Description
Rabbit monoclonal [EPR11830] to PDE4B -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cytmore details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Human
Does not react with: Rat -
Immunogen
Recombinant fragment within Human PDE4B aa 550 to the C-terminus. The exact sequence is proprietary.
Database link: Q07343 -
Positive control
- WB: HeLa, A549, Human fetal brain and Human glioma lysates; Mouse brain tissue lysate. Flow Cyt: A549 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11830 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab170939 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/5000. Predicted molecular weight: 83 kDa. | |
| Flow Cyt | 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|
Target
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Function
Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May be involved in mediating central nervous system effects of therapeutic agents ranging from antidepressants to antiasthmatic and anti-inflammatory agents. -
Tissue specificity
Expressed in brain, heart, lung and skeletal muscle. -
Pathway
Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1. -
Sequence similarities
Belongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily. - Information by UniProt
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Database links
- Entrez Gene: 5142 Human
- Entrez Gene: 18578 Mouse
- Omim: 600127 Human
- SwissProt: Q07343 Human
- Unigene: 198072 Human
- Unigene: 20181 Mouse
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Alternative names
- 5''-cyclic phosphodiesterase 4B antibody
- cAMP-specific 3'' antibody
- cAMP-specific 3',5'-cyclic phosphodiesterase 4B antibody
see all
Images
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![Western blot - Anti-PDE4B antibody [EPR11830] (ab170939)](https://www.abcam.com/ps/products/170/ab170939/Images/ab170939-393599-anti-pde4b-antibody-epr11830-western-blot-human-knockout-lysate.jpg)
Western blot - Anti-PDE4B antibody [EPR11830] (ab170939)All lanes : Anti-PDE4B antibody [EPR11830] (ab170939) at 1/500 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : PDE4B knockout HeLa cell lysate
Lane 3 : A549 cell lysate
Lane 4 : Mouse brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 83 kDa
Observed band size: 83 kDaLanes 1-4: Merged signal (red and green). Green - ab170939 observed at 83 kDa. Red - loading control ab8245 observed at 36 kDa.
ab170939 Anti-PDE4B antibody [EPR11830] was shown to specifically react with PDE4B in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265259 (knockout cell lysate ab257576) was used. Wild-type and PDE4B knockout samples were subjected to SDS-PAGE. ab170939 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-PDE4B antibody [EPR11830] (ab170939)](https://www.abcam.com/ps/products/170/ab170939/Images/ab170939-171369-anti-pde4b-antibody-epr11830-western-blot.jpg)
Western blot - Anti-PDE4B antibody [EPR11830] (ab170939)All lanes : Anti-PDE4B antibody [EPR11830] (ab170939) at 1/1000 dilution
Lane 1 : Human fetal brain lysates
Lane 2 : Human glioma lysates
Lane 3 : A549 lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 83 kDa -
![Flow Cytometry - Anti-PDE4B antibody [EPR11830] (ab170939)](https://www.abcam.com/ps/products/170/ab170939/Images/ab170939-171368-anti-pde4b-antibody-epr11830-flow-cytometry.jpg)
Flow Cytometry - Anti-PDE4B antibody [EPR11830] (ab170939)Flow cytometric analysis of permeabilized A549 cells labeling PDE4B using ab170939 (red) at 1/10 dilution, or a rabbit IgG (negative) (green).
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![Anti-PDE4B antibody [EPR11830] (ab170939)](https://www.abcam.com/ps/products/170/ab170939/Images/ab170939-4-benefits-of-recombinant-antibodies.png)
Anti-PDE4B antibody [EPR11830] (ab170939)
Datasheets and documents
References (2)
ab170939 has been referenced in 2 publications.
- Lee JW et al. Pistacia weinmannifolia ameliorates cigarette smoke and lipopolysaccharide-induced pulmonary inflammation by inhibiting interleukin-8 production and NF-?B activation. Int J Mol Med 44:949-959 (2019). PubMed: 31257455
- Wang Z et al. Curcumin restrains hepatic glucose production by blocking cAMP/PKA signaling and reducing acetyl CoA accumulation in high-fat diet (HFD)-fed mice. Mol Cell Endocrinol 474:127-136 (2018). WB ; Mouse . PubMed: 29499209
