For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    pde4b-antibody-epr11830-ab170939.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Signal Transduction Second Messenger Nucleotide Messenger cAMP
Share by email
Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-PDE4B antibody [EPR11830] (ab170939)

  • Datasheet
Submit a review Submit a question References (2)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-PDE4B antibody [EPR11830] (ab170939)
  • Western blot - Anti-PDE4B antibody [EPR11830] (ab170939)
  • Flow Cytometry (Intracellular) - Anti-PDE4B antibody [EPR11830] (ab170939)
  • Anti-PDE4B antibody [EPR11830] (ab170939)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11830] to PDE4B
  • Suitable for: Flow Cyt (Intra), WB
  • Knockout validated
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Carrier Free

You may also be interested in

Primary
Product image
Anti-Fyn (phospho Y530) + Yes (phospho Y537) antibody [EPR13512] (ab188319)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Primary
Product image
FITC Anti-PD-L1 antibody [28-8] (ab224027)

View more associated products

Overview

  • Product name

    Anti-PDE4B antibody [EPR11830]
    See all PDE4B primary antibodies
  • Description

    Rabbit monoclonal [EPR11830] to PDE4B
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt (Intra), WBmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Human
    Does not react with: Rat
  • Immunogen

    Recombinant fragment within Human PDE4B aa 550 to the C-terminus. The exact sequence is proprietary.
    Database link: Q07343

  • Positive control

    • WB: HeLa, A549, Human fetal brain and Human glioma lysates; Mouse brain tissue lysate. Flow Cyt (intra): A549 cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR11830
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Second Messenger
    • Nucleotide Messenger
    • cAMP

Associated products

  • Alternative Versions

    • Anti-PDE4B antibody [EPR11830] - BSA and Azide free (ab249608)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human PDE4B knockout HeLa cell line (ab265259)
  • KO cell lysates

    • Human PDE4B knockout HeLa cell lysate (ab257576)
  • Positive Controls

    • A549 whole cell lysate (ab7910)
  • Recombinant Protein

    • Recombinant human PDE4B protein (ab56612)
  • Related Products

    • Recombinant human PDE4B protein (ab56612)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab170939 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB
1/1000 - 1/5000. Predicted molecular weight: 83 kDa.
Notes
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB
1/1000 - 1/5000. Predicted molecular weight: 83 kDa.
Application notes
Is unsuitable for ICC/IF,IHC-P or IP.

Target

  • Function

    Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May be involved in mediating central nervous system effects of therapeutic agents ranging from antidepressants to antiasthmatic and anti-inflammatory agents.
  • Tissue specificity

    Expressed in brain, heart, lung and skeletal muscle.
  • Pathway

    Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.
  • Sequence similarities

    Belongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily.
  • Target information above from: UniProt accession Q07343 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 5142 Human
    • Entrez Gene: 18578 Mouse
    • Omim: 600127 Human
    • SwissProt: Q07343 Human
    • Unigene: 198072 Human
    • Alternative names

      • 5''-cyclic phosphodiesterase 4B antibody
      • cAMP-specific 3'' antibody
      • cAMP-specific 3',5'-cyclic phosphodiesterase 4B antibody
      • cAMP-specific phosphodiesterase-4 B isoform antibody
      • DKFZp686F2182 antibody
      • DPDE4 antibody
      • dunce-like phosphodiesterase E4 antibody
      • MGC126529 antibody
      • OTTHUMP00000010652 antibody
      • OTTHUMP00000010653 antibody
      • OTTHUMP00000010654 antibody
      • OTTHUMP00000010656 antibody
      • OTTHUMP00000232365 antibody
      • PDE 4B antibody
      • PDE IVB antibody
      • PDE32 antibody
      • Pde4b antibody
      • PDE4B_HUMAN antibody
      • PDE4B5 antibody
      • PDEIVB antibody
      • Phosphodiesterase 4B antibody
      • Phosphodiesterase 4B cAMP specific antibody
      • Phosphodiesterase 4B, cAMP specific (phosphodiesterase E4 dunce homolog, Drosophila) antibody
      see all

    Images

    • Western blot - Anti-PDE4B antibody [EPR11830] (ab170939)
      Western blot - Anti-PDE4B antibody [EPR11830] (ab170939)
      All lanes : Anti-PDE4B antibody [EPR11830] (ab170939) at 1/500 dilution

      Lane 1 : Wild-type HeLa cell lysate
      Lane 2 : PDE4B knockout HeLa cell lysate
      Lane 3 : A549 cell lysate
      Lane 4 : Mouse brain tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

      Predicted band size: 83 kDa
      Observed band size: 83 kDa



      Lanes 1-4: Merged signal (red and green). Green - ab170939 observed at 83 kDa. Red - loading control ab8245 observed at 36 kDa. 

       ab170939 Anti-PDE4B antibody [EPR11830] was shown to specifically react with PDE4B in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265259 (knockout cell lysate ab257576) was used.  Wild-type and PDE4B knockout samples were subjected to SDS-PAGE.  ab170939 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively.  Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    • Western blot - Anti-PDE4B antibody [EPR11830] (ab170939)
      Western blot - Anti-PDE4B antibody [EPR11830] (ab170939)
      All lanes : Anti-PDE4B antibody [EPR11830] (ab170939) at 1/1000 dilution

      Lane 1 : Human fetal brain lysates
      Lane 2 : Human glioma lysates
      Lane 3 : A549 lysates

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 83 kDa

    • Flow Cytometry (Intracellular) - Anti-PDE4B antibody [EPR11830] (ab170939)
      Flow Cytometry (Intracellular) - Anti-PDE4B antibody [EPR11830] (ab170939)

      Intracellular flow cytometric analysis of permeabilized A549 cells labeling PDE4B using ab170939 (red)at 1/10 dilution,or a rabbit IgG (negative) (green). 

       

       

    • Anti-PDE4B antibody [EPR11830] (ab170939)
      Anti-PDE4B antibody [EPR11830] (ab170939)

    Protocols

    • Western blot protocols
    • Flow cytometry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (2)

    Publishing research using ab170939? Please let us know so that we can cite the reference in this datasheet.

    ab170939 has been referenced in 2 publications.

    • Lee JW  et al. Pistacia weinmannifolia ameliorates cigarette smoke and lipopolysaccharide-induced pulmonary inflammation by inhibiting interleukin-8 production and NF-?B activation. Int J Mol Med 44:949-959 (2019). PubMed: 31257455
    • Wang Z  et al. Curcumin restrains hepatic glucose production by blocking cAMP/PKA signaling and reducing acetyl CoA accumulation in high-fat diet (HFD)-fed mice. Mol Cell Endocrinol 474:127-136 (2018). WB ; Mouse . PubMed: 29499209

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab170939.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2022 Abcam plc. All rights reserved.