Anti-PDE4B antibody [EPR11830] (ab170939)
- Datasheet
- References (1)
- Protocols
Overview
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Product nameAnti-PDE4B antibody [EPR11830]
See all PDE4B primary antibodies -
DescriptionRabbit monoclonal [EPR11830] to PDE4B
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Host speciesRabbit
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Tested applicationsSuitable for: WB, Flow Cytmore details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivityReacts with: Mouse, Human
Does not react with: Rat -
Immunogen
Recombinant fragment within Human PDE4B aa 550 to the C-terminus. The exact sequence is proprietary.
Database link: Q07343 -
Positive control
- Human fetal brain, Human glioma and A549 lysates; A549 cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEPR11830
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IsotypeIgG
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Research areas
Associated products
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Isotype control
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Positive Controls
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Related Products
Applications
Our Abpromise guarantee covers the use of ab170939 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/5000. Predicted molecular weight: 83 kDa. | |
| Flow Cyt | 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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Target
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FunctionHydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May be involved in mediating central nervous system effects of therapeutic agents ranging from antidepressants to antiasthmatic and anti-inflammatory agents.
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Tissue specificityExpressed in brain, heart, lung and skeletal muscle.
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PathwayPurine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.
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Sequence similaritiesBelongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily.
- Information by UniProt
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Database links
- Entrez Gene: 5142 Human
- Entrez Gene: 18578 Mouse
- Omim: 600127 Human
- SwissProt: Q07343 Human
- Unigene: 198072 Human
- Unigene: 20181 Mouse
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Alternative names
- 5''-cyclic phosphodiesterase 4B antibody
- cAMP-specific 3'' antibody
- cAMP-specific 3',5'-cyclic phosphodiesterase 4B antibody
see all
Images
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![Western blot - Anti-PDE4B antibody [EPR11830] (ab170939)](https://www.abcam.com/ps/products/170/ab170939/Images/ab170939-171369-79091wb22012medium.jpg)
Western blot - Anti-PDE4B antibody [EPR11830] (ab170939)All lanes : Anti-PDE4B antibody [EPR11830] (ab170939) at 1/1000 dilution
Lane 1 : Human fetal brain lysates
Lane 2 : Human glioma lysates
Lane 3 : A549 lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 83 kDa -
![Flow Cytometry - Anti-PDE4B antibody [EPR11830] (ab170939)](https://www.abcam.com/ps/products/170/ab170939/Images/ab170939-171368-79091fc22014medium.jpg)
Flow Cytometry - Anti-PDE4B antibody [EPR11830] (ab170939)Flow cytometric analysis of permeabilized A549 cells labeling PDE4B using ab170939 (red) at 1/10 dilution, or a rabbit IgG (negative) (green).
Datasheets and documents
References
This product has been referenced in:
- Wang Z et al. Curcumin restrains hepatic glucose production by blocking cAMP/PKA signaling and reducing acetyl CoA accumulation in high-fat diet (HFD)-fed mice. Mol Cell Endocrinol 474:127-136 (2018). WB ; Mouse . Read more (PubMed: 29499209) »
