Recombinant Anti-PDE4B antibody [EPR11830] (ab170939)
![Western blot - Anti-PDE4B antibody [EPR11830] (ab170939)](https://www.abcam.com/ps/products/170/ab170939/Images/ab170939-393599-anti-pde4b-antibody-epr11830-western-blot-human-knockout-lysate.jpg "Western blot - Anti-PDE4B antibody [EPR11830] (ab170939)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11830] to PDE4B
- Suitable for: Flow Cyt (Intra), WB
- Knockout validated
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-PDE4B antibody [EPR11830]
See all PDE4B primary antibodies -
Description
Rabbit monoclonal [EPR11830] to PDE4B -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WBmore details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Human
Does not react with: Rat -
Immunogen
Recombinant fragment within Human PDE4B aa 550 to the C-terminus. The exact sequence is proprietary.
Database link: Q07343 -
Positive control
- WB: HeLa, A549, Human fetal brain and Human glioma lysates; Mouse brain tissue lysate. Flow Cyt (intra): A549 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11830 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab170939 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB |
1/1000 - 1/5000. Predicted molecular weight: 83 kDa.
|
| Notes |
|---|
|
Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/1000 - 1/5000. Predicted molecular weight: 83 kDa. |
Target
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Function
Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May be involved in mediating central nervous system effects of therapeutic agents ranging from antidepressants to antiasthmatic and anti-inflammatory agents. -
Tissue specificity
Expressed in brain, heart, lung and skeletal muscle. -
Pathway
Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1. -
Sequence similarities
Belongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily. - Information by UniProt
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Database links
- Entrez Gene: 5142 Human
- Entrez Gene: 18578 Mouse
- Omim: 600127 Human
- SwissProt: Q07343 Human
- Unigene: 198072 Human
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Alternative names
- 5''-cyclic phosphodiesterase 4B antibody
- cAMP-specific 3'' antibody
- cAMP-specific 3',5'-cyclic phosphodiesterase 4B antibody
see all
Images
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Western blot - Anti-PDE4B antibody [EPR11830] (ab170939)All lanes : Anti-PDE4B antibody [EPR11830] (ab170939) at 1/500 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : PDE4B knockout HeLa cell lysate
Lane 3 : A549 cell lysate
Lane 4 : Mouse brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 83 kDa
Observed band size: 83 kDaLanes 1-4: Merged signal (red and green). Green - ab170939 observed at 83 kDa. Red - loading control ab8245 observed at 36 kDa.
ab170939 Anti-PDE4B antibody [EPR11830] was shown to specifically react with PDE4B in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265259 (knockout cell lysate ab257576) was used. Wild-type and PDE4B knockout samples were subjected to SDS-PAGE. ab170939 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-PDE4B antibody [EPR11830] (ab170939)](https://www.abcam.com/ps/products/170/ab170939/Images/ab170939-171369-anti-pde4b-antibody-epr11830-western-blot.jpg)
Western blot - Anti-PDE4B antibody [EPR11830] (ab170939)All lanes : Anti-PDE4B antibody [EPR11830] (ab170939) at 1/1000 dilution
Lane 1 : Human fetal brain lysates
Lane 2 : Human glioma lysates
Lane 3 : A549 lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 83 kDa -
![Flow Cytometry (Intracellular) - Anti-PDE4B antibody [EPR11830] (ab170939)](https://www.abcam.com/ps/products/170/ab170939/Images/ab170939-171368-anti-pde4b-antibody-epr11830-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-PDE4B antibody [EPR11830] (ab170939)Intracellular flow cytometric analysis of permeabilized A549 cells labeling PDE4B using ab170939 (red)at 1/10 dilution,or a rabbit IgG (negative) (green).
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![Anti-PDE4B antibody [EPR11830] (ab170939)](https://www.abcam.com/ps/products/170/ab170939/Images/ab170939-4-benefits-of-recombinant-antibodies.png)
Anti-PDE4B antibody [EPR11830] (ab170939)
Datasheets and documents
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Datasheet download
References (2)
ab170939 has been referenced in 2 publications.
- Lee JW et al. Pistacia weinmannifolia ameliorates cigarette smoke and lipopolysaccharide-induced pulmonary inflammation by inhibiting interleukin-8 production and NF-?B activation. Int J Mol Med 44:949-959 (2019). PubMed: 31257455
- Wang Z et al. Curcumin restrains hepatic glucose production by blocking cAMP/PKA signaling and reducing acetyl CoA accumulation in high-fat diet (HFD)-fed mice. Mol Cell Endocrinol 474:127-136 (2018). WB ; Mouse . PubMed: 29499209
