Recombinant
RabMAb

Recombinant Anti-PDE4B antibody [EPR11830] (ab170939)

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Overview

  • Product name

    Anti-PDE4B antibody [EPR11830]
    See all PDE4B primary antibodies

  • Description

    Rabbit monoclonal [EPR11830] to PDE4B

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC/IF,IHC-P or IP

  • Species reactivity

    Reacts with: Mouse, Human
    Does not react with: Rat

  • Immunogen

    Recombinant fragment within Human PDE4B aa 550 to the C-terminus. The exact sequence is proprietary.
    Database link: Q07343

  • Positive control

    • Human fetal brain, Human glioma and A549 lysates; A549 cells.

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR11830

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab170939 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 83 kDa.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.

    • Target

    • Function

      Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May be involved in mediating central nervous system effects of therapeutic agents ranging from antidepressants to antiasthmatic and anti-inflammatory agents.

    • Tissue specificity

      Expressed in brain, heart, lung and skeletal muscle.

    • Pathway

      Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.

    • Sequence similarities

      Belongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily.
    • Information by UniProt

    • Database links

      • Alternative names

        • 5''-cyclic phosphodiesterase 4B antibody
        • cAMP-specific 3'' antibody
        • cAMP-specific 3',5'-cyclic phosphodiesterase 4B antibody
        • cAMP-specific phosphodiesterase-4 B isoform antibody
        • DKFZp686F2182 antibody
        • DPDE4 antibody
        • dunce-like phosphodiesterase E4 antibody
        • MGC126529 antibody
        • OTTHUMP00000010652 antibody
        • OTTHUMP00000010653 antibody
        • OTTHUMP00000010654 antibody
        • OTTHUMP00000010656 antibody
        • OTTHUMP00000232365 antibody
        • PDE 4B antibody
        • PDE IVB antibody
        • PDE32 antibody
        • Pde4b antibody
        • PDE4B_HUMAN antibody
        • PDE4B5 antibody
        • PDEIVB antibody
        • Phosphodiesterase 4B antibody
        • Phosphodiesterase 4B cAMP specific antibody
        • Phosphodiesterase 4B, cAMP specific (phosphodiesterase E4 dunce homolog, Drosophila) antibody
        see all

      Images

      • Western blot - Anti-PDE4B antibody [EPR11830] (ab170939)
        Western blot - Anti-PDE4B antibody [EPR11830] (ab170939)
        All lanes : Anti-PDE4B antibody [EPR11830] (ab170939) at 1/1000 dilution

        Lane 1 : Human fetal brain lysates
        Lane 2 : Human glioma lysates
        Lane 3 : A549 lysates

        Lysates/proteins at 10 µg per lane.

        Predicted band size: 83 kDa

      • Flow Cytometry - Anti-PDE4B antibody [EPR11830] (ab170939)
        Flow Cytometry - Anti-PDE4B antibody [EPR11830] (ab170939)

        Flow cytometric analysis of permeabilized A549 cells labeling PDE4B using ab170939 (red) at 1/10 dilution, or a rabbit IgG (negative) (green).

      References

      This product has been referenced in:

      • Lee JW  et al. Pistacia weinmannifolia ameliorates cigarette smoke and lipopolysaccharide-induced pulmonary inflammation by inhibiting interleukin-8 production and NF-?B activation. Int J Mol Med 44:949-959 (2019). Read more (PubMed: 31257455) »
      • Wang Z  et al. Curcumin restrains hepatic glucose production by blocking cAMP/PKA signaling and reducing acetyl CoA accumulation in high-fat diet (HFD)-fed mice. Mol Cell Endocrinol 474:127-136 (2018). WB ; Mouse . Read more (PubMed: 29499209) »
      See all 2 Publications for this product

      Publishing research using ab170939? Please let us know so that we can cite the reference in this datasheet.

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