Anti-PDE4C antibody (ab97356)
Key features and details
- Rabbit polyclonal to PDE4C
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-PDE4C antibody
See all PDE4C primary antibodies -
Description
Rabbit polyclonal to PDE4C -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Pig
-
Immunogen
Recombinant protein fragment containing a sequence corresponding to a region within amino acids 325 and 637 of Human PDE4C (NP_000914).
-
Positive control
- 293T whole cell lysate, A431, HeLaS3, and Raji cell lysates.
-
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
Our Abpromise guarantee covers the use of ab97356 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 80 kDa. |
Target
-
Function
Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. -
Tissue specificity
Expressed in various tissues but not in cells of the immune system. -
Pathway
Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1. -
Sequence similarities
Belongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily. - Information by UniProt
-
Database links
- Entrez Gene: 5143 Human
- Entrez Gene: 110385 Mouse
- Entrez Gene: 290646 Rat
- Omim: 600128 Human
- SwissProt: Q08493 Human
- SwissProt: Q3UEI1 Mouse
- SwissProt: P14644 Rat
- Unigene: 132584 Human
see all -
Alternative names
- 5''-cyclic phosphodiesterase 4C antibody
- cAMP specific 3' 5' cyclic phosphodiesterase 4C antibody
- cAMP-specific 3'' antibody
see all
Images
-
Western blot - Anti-PDE4C antibody (ab97356)Anti-PDE4C antibody (ab97356) at 1/1000 dilution + 293T whole cell lysate at 30 µg
Predicted band size: 80 kDa
7.5% SDS PAGE
References (1)
ab97356 has been referenced in 1 publication.
- Fang Q et al. PGE 2 desensitizes ß -agonist effect on human lung fibroblast-mediated collagen gel contraction through upregulating PDE4. Mediators Inflamm 2013:145197 (2013). PubMed: 24227907
