Overview

  • Product name

  • Description

    Goat polyclonal to PDE4D
  • Host species

    Goat
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide:

    QPEACVIDDRSPDT

    , corresponding to C terminal amino acids 670-673 of Human PDE4D (NP_006194.2).

  • Positive control

    • A549 cell lysate. PDE4D transfected COS-7 lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 0.5% BSA, 0.5% Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab77080 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunising peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab77080 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 91 kDa.

Target

  • Function

    Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.
  • Tissue specificity

    Widespread; most abundant in skeletal muscle. Isoform 6 is detected in brain. Isoform 8 is detected in brain, placenta, lung and kidney. Isoform 7 is detected in heart and skeletal muscle.
  • Pathway

    Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.
  • Involvement in disease

    Note=Genetic variations in PDE4D might be associated with susceptibility to stroke. PubMed:17006457 states that association with stroke has to be considered with caution.
  • Sequence similarities

    Belongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily.
  • Post-translational
    modifications

    Isoform 3 and isoform 7 are activated by phosphorylation (in vitro), but not isoform 6. Isoform N3 and isoform 12 are phosphorylated on Ser-49, Ser-51, Ser-55 and Ser-59.
  • Cellular localization

    Cytoplasm. Membrane. Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Found in the soluble fraction, associated with membranes, and associated with the cytoskeleton and the centrosome.
  • Information by UniProt
  • Database links

  • Alternative names

    • cAMP specific 3',5' cyclic phosphodiesterase 4D antibody
    • DKFZp686M11213 antibody
    • 5''-cyclic phosphodiesterase 4D antibody
    • cAMP specific phosphodiesterase 4D antibody
    • cAMP specific phosphodiesterase PDE4D6 antibody
    • cAMP-specific 3'' antibody
    • DPDE3 antibody
    • Dunce like phosphodiesterase E3 antibody
    • FLJ97311 antibody
    • HSPDE4D antibody
    • PDE43 antibody
    • Pde4d antibody
    • PDE4D_HUMAN antibody
    • PDE4DN2 antibody
    • Phosphodiesterase 4D cAMP specific (dunce (Drosophila) homolog phosphodiesterase E3) antibody
    • Phosphodiesterase 4D cAMP specific (phosphodiesterase E3 dunce homolog Drosophila) antibody
    • Phosphodiesterase 4D cAMP specific antibody
    • Phosphodiesterase 4D, cAMP specific (dunce) antibody
    • STRK1 antibody
    see all

Images

  • All lanes : Anti-PDE4D antibody (ab77080) at 1 µg/ml

    Lane 1 : COS cell lysates transfected with Human PDE4D isoform 1
    Lane 2 : COS cell lysates transfected with Human PDE4D isoform 3
    Lane 3 : COS cell lysates, non-transfected

    Lysates/proteins at 25 µg per lane.

    Predicted band size: 91 kDa
    Observed band size: 68,95 kDa
    why is the actual band size different from the predicted?



    Primary incubation was 1 hour. Detected by chemiluminescence.
  • Anti-PDE4D antibody (ab77080) at 0.3 µg/ml + A549 lysate in RIPA buffer at 35 µg

    Predicted band size: 91 kDa
    Observed band size: 70,80,90 kDa why is the actual band size different from the predicted?



    Primary incubation was 1 hour. Detected by chemiluminescence.
  • Anti-PDE4D antibody (ab77080) at 0.3 µg/ml + lysates prepared from transfected HEK293 cells, transiently expressing PDE4D

    Predicted band size: 91 kDa



    Multiple bands from ~55-80kDa are observed.
    These bands are not observed in the non-transfected HEK293.

References

This product has been referenced in:

  • Bazhin AV  et al. Distinct metabolism of cyclic adenosine monophosphate in regulatory and helper CD4+ T cells. Mol Immunol 47:678-84 (2010). ELISA . Read more (PubMed: 19939455) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for contacting us. I apologise for the delay, I wanted to check this case with my lab colleagues.
Regarding ab59249, the amino acid range of this immunogen is aaXXXX of human PDE4D with accession# Q08499 (http://www.uniprot.org/uniprot/Q08499).
This antibody binds to isoforms 4, 3, 5, N3, 8, 9, 7, and 12. It does not bind to isoform 10, 1, 2, and 6. According to the isoforms that this antibody binds, you would expect to see two bands, approximately 25 and 85 kDa. Because the western blot on our website is cut off at 34 kDa, we can retest this antibody to see if it does actually bind the lower isoform as well.
Although we haven’t specifically tested the antibody against all isoforms, there are other alternatives that may also recognise several forms of the protein:
For ab77080 we ran a BLAST search and found the antigen sequence in all of 9 isoforms:
https://www.abcam.com/PDE4D-antibody-ab77080.html
Ab112018 also shares 100% identity to isoforms PDE4D1-9:
https://www.abcam.com/PDE4D-antibody-ab112018.html
Ab99409 and ab87329 recognize 10 of 12 isoforms listed for SwissProt entry Q08499. The two isoforms listed for SwissProt Q08499 not recognized by either of them are sp|Q08499-7|PDE4D_HUMAN and sp|Q08499-12|PDE4D_HUMAN:
http://www.uniprot.org/uniprot/Q08499
And ab87329’s immunogen is present in 8 out of 12 of them so it should detect isoforms 1- 10.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Answer

Thank you for contacting us and sorry for the delay in response. I now have confirmation of the specificity of the two antibodies in question. ab14613 has been raised against a C-terminal peptide sequence of PDE4D. This peptide, contrary to what was published on the datasheet should recognise all 9 isoforms. I am sorry for this misinformation. This is as a result of science moving on and new isoforms being identified. This will now be updated on the datasheet. Similarly, ab77080 has been raised against the C-terminal amino acids (QPEACVIDDRSPDT) of human PDE4D this should also detect all 9 isoforms of PDE4D. If you have any further questions please do not hesitate to ask.

Read More

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