Recombinant Anti-PDE4D antibody [EPR11823-31] (ab171750)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11823-31] to PDE4D
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-PDE4D antibody [EPR11823-31]
See all PDE4D primary antibodies -
Description
Rabbit monoclonal [EPR11823-31] to PDE4D -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide within Human PDE4D aa 650 to the C-terminus. The exact sequence is proprietary.
Database link: Q08499 -
Positive control
- Human fetal heart and fetal brain lysates.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
-
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11823-31 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab171750 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (1) |
1/1000 - 1/5000. Predicted molecular weight: 91 kDa.
|
Notes |
---|
WB
1/1000 - 1/5000. Predicted molecular weight: 91 kDa. |
Target
-
Function
Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. -
Tissue specificity
Widespread; most abundant in skeletal muscle. Isoform 6 is detected in brain. Isoform 8 is detected in brain, placenta, lung and kidney. Isoform 7 is detected in heart and skeletal muscle. -
Pathway
Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1. -
Involvement in disease
Note=Genetic variations in PDE4D might be associated with susceptibility to stroke. PubMed:17006457 states that association with stroke has to be considered with caution. -
Sequence similarities
Belongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily. -
Post-translational
modificationsIsoform 3 and isoform 7 are activated by phosphorylation (in vitro), but not isoform 6. Isoform N3 and isoform 12 are phosphorylated on Ser-49, Ser-51, Ser-55 and Ser-59. -
Cellular localization
Cytoplasm. Membrane. Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Found in the soluble fraction, associated with membranes, and associated with the cytoskeleton and the centrosome. - Information by UniProt
-
Database links
- Entrez Gene: 5144 Human
- Entrez Gene: 238871 Mouse
- Entrez Gene: 24627 Rat
- Omim: 600129 Human
- SwissProt: Q08499 Human
- SwissProt: Q01063 Mouse
- SwissProt: P14270 Rat
- Unigene: 117545 Human
see all -
Alternative names
- cAMP specific 3',5' cyclic phosphodiesterase 4D antibody
- DKFZp686M11213 antibody
- 5''-cyclic phosphodiesterase 4D antibody
see all
Images
Datasheets and documents
-
SDS download
-
Datasheet download
References (7)
ab171750 has been referenced in 7 publications.
- Yuan F et al. Effect of phosphodiesterase-4 inhibitor rolipram on colonic hypermotility in water avoidance stress rat model. Neurogastroenterol Motil 34:e14317 (2022). PubMed: 35037375
- Zhang H et al. Synergistic effects of two naturally occurring iridoids in eliciting a rapid antidepressant action by up-regulating hippocampal PACAP signalling. Br J Pharmacol 179:4078-4091 (2022). PubMed: 35362097
- Varona S et al. Rolipram Prevents the Formation of Abdominal Aortic Aneurysm (AAA) in Mice: PDE4B as a Target in AAA. Antioxidants (Basel) 10:N/A (2021). PubMed: 33809405
- Li W et al. MicroRNA-30d-5p ameliorates lipopolysaccharide-induced acute lung injury via activating AMPKa. Immunopharmacol Immunotoxicol 43:431-442 (2021). PubMed: 34157933
- Abou Saleh L et al. Ablation of PDE4B protects from Pseudomonas aeruginosa-induced acute lung injury in mice by ameliorating the cytostorm and associated hypothermia. FASEB J 35:e21797 (2021). PubMed: 34383981
- Zhang B et al. Arecoline Enhances Phosphodiesterase 4A Activity to Promote Transforming Growth Factor-β-Induced Buccal Mucosal Fibroblast Activation via cAMP-Epac1 Signaling Pathway. Front Pharmacol 12:722040 (2021). PubMed: 34819854
- Lupieri A et al. A non-catalytic function of PI3K? drives smooth muscle cell proliferation after arterial damage. J Cell Sci 133:N/A (2020). PubMed: 32482794