Key features and details
- Rabbit polyclonal to PDE4D (phospho S190)
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PDE4D (phospho S190) antibody
See all PDE4D primary antibodies
DescriptionRabbit polyclonal to PDE4D (phospho S190)
Specificityab59212 detects endogenous levels of PDE4D only when phosphorylated at serine 190.
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic phosphopeptide derived from human PDE4D around the phosphorylation site of serine 190 (R-E-SP-F-L).
- IHC-P: Human colon carcinoma tissue WB: K562 cell extracts treated with H2O2 (100uM, 30mins).
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg+2 and Ca+2
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab59212 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
Our Abpromise guarantee covers the use of ab59212 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/100.|
|WB||1/500 - 1/1000. Detects a band of approximately 75 kDa (predicted molecular weight: 91 kDa).|
FunctionHydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.
Tissue specificityWidespread; most abundant in skeletal muscle. Isoform 6 is detected in brain. Isoform 8 is detected in brain, placenta, lung and kidney. Isoform 7 is detected in heart and skeletal muscle.
PathwayPurine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.
Involvement in diseaseNote=Genetic variations in PDE4D might be associated with susceptibility to stroke. PubMed:17006457 states that association with stroke has to be considered with caution.
Sequence similaritiesBelongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily.
modificationsIsoform 3 and isoform 7 are activated by phosphorylation (in vitro), but not isoform 6. Isoform N3 and isoform 12 are phosphorylated on Ser-49, Ser-51, Ser-55 and Ser-59.
Cellular localizationCytoplasm. Membrane. Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Found in the soluble fraction, associated with membranes, and associated with the cytoskeleton and the centrosome.
- Information by UniProt
- cAMP specific 3',5' cyclic phosphodiesterase 4D antibody
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All lanes : Anti-PDE4D (phospho S190) antibody (ab59212) at 1/500 dilution
Lane 1 : K562 cell extract treated
with H2O2 (100uM, 30mins)
Lane 2 : K562 cell extract treated
with H2O2 (100uM, 30mins) with immunising peptide
Predicted band size: 91 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
ab59212, at 1/50 dilution, staining PDE4D in paraffin embedded human colon carcinoma tissue by Immunohistochemistry in the absence or presence of the immunising peptide.
ab59212 has been referenced in 2 publications.
- Wilson NM et al. Traumatic Brain Injury Upregulates Phosphodiesterase Expression in the Hippocampus. Front Syst Neurosci 10:5 (2016). WB . PubMed: 26903822
- Oliva AA et al. Phosphodiesterase isoform-specific expression induced by traumatic brain injury. J Neurochem 123:1019-29 (2012). PubMed: 23057870