Anti-PDE5A/PDE5 antibody (ab14672)
Key features and details
- Rabbit polyclonal to PDE5A/PDE5
- Suitable for: IHC-P, IP, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-PDE5A/PDE5 antibody
See all PDE5A/PDE5 primary antibodies -
Description
Rabbit polyclonal to PDE5A/PDE5 -
Host species
Rabbit -
Specificity
This antibody detects 99 and 89 kDa PDE5A/PDE5 proteins in various tissues.
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Tested applications
Suitable for: IHC-P, IP, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human PDE5A/PDE5. Synthetic peptide common to all PDE5A variants, taken within amino acid region 120-170 of human PDE5A protein.
(Peptide available asab150691) -
General notes
Storage of very dilute antibody solutions is not recommended.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.02% Sodium azide -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified on antigen sepharose affinity column. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab14672 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | (1) |
1/10. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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IP |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration.
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ICC/IF |
Use a concentration of 1 µg/ml.
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Notes |
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IHC-P
1/10. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
IP
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. |
ICC/IF
Use a concentration of 1 µg/ml. |
Target
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Function
Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. This phosphodiesterase catalyzes the specific hydrolysis of cGMP to 5'-GMP. -
Tissue specificity
Expressed in aortic smooth muscle cells, heart, placenta, skeletal muscle and pancreas and, to a much lesser extent, in brain, liver and lung. -
Pathway
Purine metabolism; 3',5'-cyclic GMP degradation; GMP from 3',5'-cyclic GMP: step 1/1. -
Sequence similarities
Belongs to the cyclic nucleotide phosphodiesterase family.
Contains 2 GAF domains. -
Domain
Composed of a C-terminal catalytic domain containing two putative divalent metal sites and an N-terminal regulatory domain which contains two homologous allosteric cGMP-binding regions, A and B. -
Post-translational
modificationsPhosphorylation is regulated by binding of cGMP to the two allosteric sites. - Information by UniProt
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Database links
- Entrez Gene: 8654 Human
- Entrez Gene: 242202 Mouse
- Entrez Gene: 171115 Rat
- Omim: 603310 Human
- SwissProt: O76074 Human
- SwissProt: Q8CG03 Mouse
- SwissProt: O54735 Rat
- Unigene: 647971 Human
see all -
Alternative names
- 5''-cyclic phosphodiesterase antibody
- CGB PDE antibody
- CGB-PDE antibody
see all
Images
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ab14672 (4µg/ml) staining PDE5A/PDE5 in human oesophagus using an automated system (DAKO Autostainer Plus). Using this protocol there is staining of the cytoplasm and nuclei in stratified squamous epithelium cells.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required. -
ICC/IF image of ab14672 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14672, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
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Datasheet download
References (12)
ab14672 has been referenced in 12 publications.
- Kabwe JC et al. CRISPR-mediated Bmpr2 point mutation exacerbates late pulmonary vasculopathy and reduces survival in rats with experimental pulmonary hypertension. Respir Res 23:87 (2022). PubMed: 35395852
- Claveria-Cabello A et al. Dual Pharmacological Targeting of HDACs and PDE5 Inhibits Liver Disease Progression in a Mouse Model of Biliary Inflammation and Fibrosis. Cancers (Basel) 12:N/A (2020). PubMed: 33322158
- Reheman A et al. Optimization of Extraction Technology of Majun Mupakhi Ela and its Effect on Hydrocortisone-induced Kidney Yang Deficiency in Mice. Sci Rep 9:4628 (2019). PubMed: 30874604
- Jin S et al. Activation of cGMP/PKG/p65 signaling associated with PDE5-Is downregulates CCL5 secretion by CD8 + T cells in benign prostatic hyperplasia. Prostate 79:909-919 (2019). PubMed: 30958912
- Jia T et al. Cinaciguat in combination with insulin induces a favorable effect on implant osseointegration in type 2 diabetic rats. Biomed Pharmacother 118:109216 (2019). PubMed: 31319371