Anti-PDE5A/PDE5 antibody (ab224232)
Key features and details
- Rabbit polyclonal to PDE5A/PDE5
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PDE5A/PDE5 antibody
See all PDE5A/PDE5 primary antibodies -
Description
Rabbit polyclonal to PDE5A/PDE5 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Dog -
Immunogen
Recombinant fragment corresponding to Human PDE5A/PDE5 aa 300-450.
Database link: O76074 -
Positive control
- IHC-P: Human duodenum, pancreatic, and prostate tissue. ICC/IF: U-2 OS cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab224232 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
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IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. This phosphodiesterase catalyzes the specific hydrolysis of cGMP to 5'-GMP. -
Tissue specificity
Expressed in aortic smooth muscle cells, heart, placenta, skeletal muscle and pancreas and, to a much lesser extent, in brain, liver and lung. -
Pathway
Purine metabolism; 3',5'-cyclic GMP degradation; GMP from 3',5'-cyclic GMP: step 1/1. -
Sequence similarities
Belongs to the cyclic nucleotide phosphodiesterase family.
Contains 2 GAF domains. -
Domain
Composed of a C-terminal catalytic domain containing two putative divalent metal sites and an N-terminal regulatory domain which contains two homologous allosteric cGMP-binding regions, A and B. -
Post-translational
modificationsPhosphorylation is regulated by binding of cGMP to the two allosteric sites. - Information by UniProt
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Database links
- Entrez Gene: 281972 Cow
- Entrez Gene: 403825 Dog
- Entrez Gene: 8654 Human
- Entrez Gene: 242202 Mouse
- Entrez Gene: 171115 Rat
- Omim: 603310 Human
- SwissProt: Q28156 Cow
- SwissProt: O77746 Dog
see all -
Alternative names
- 5''-cyclic phosphodiesterase antibody
- CGB PDE antibody
- CGB-PDE antibody
see all
Images
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Paraffin-embedded human pancreas stained for PDE5A using ab224232 at 1/200 dilution in immunohistochemical analysis. Shows moderate cytoplasmic positivity in intercalacted ducts.
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Paraffin-embedded human duodenum stained for PDE5A using ab224232 at 1/200 dilution in immunohistochemical analysis. Shows weak to moderate cytoplasmic positivity in glandular cells.
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Paraffin-embedded human proostate stained for PDE5A using ab224232 at 1/200 dilution in immunohistochemical analysis. Shows weak to moderate cytoplasmic positivity in smooth muscle cells.
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Paraffin-embedded human liver stained for PDE5A using ab224232 at 1/200 dilution in immunohistochemical analysis. Shows no positivity in hepatocytes as expected.
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PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for PDE5A/PDE5 (green) using ab224232 (4 µg/ml) in ICC/IF.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab224232 has not yet been referenced specifically in any publications.