Anti-PDE5A/PDE5 antibody (ab28761)

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Overview

  • Product name

    Anti-PDE5A/PDE5 antibody
    See all PDE5A/PDE5 primary antibodies

  • Description

    Goat polyclonal to PDE5A/PDE5

  • Host species

    Goat

  • Specificity

    This antibody is expected to recognise all 4 reported isoforms (as represented by NP_001074; NP_236914; NP_246273). [NP_237223 has been retracted].

  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cytmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rat, Cow, Dog

  • Immunogen

    Synthetic peptide corresponding to Human PDE5A/PDE5 aa 864-875 (C terminal).
    Sequence:

    C-LINGESG QAKRN


    (Peptide available as ab45684)

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Human lung lysate. Lysate prepared from transfected HEK293 cells transiently expressing PDE5A/PDE5

  • General notes

     This product was previously labelled as PDE5A

     

Properties

Applications

Our Abpromise guarantee covers the use of ab28761 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 100 kDa).
ICC/IF Use a concentration of 10 µg/ml.
Flow Cyt Use a concentration of 10 µg/ml.

Target

  • Function

    Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. This phosphodiesterase catalyzes the specific hydrolysis of cGMP to 5'-GMP.

  • Tissue specificity

    Expressed in aortic smooth muscle cells, heart, placenta, skeletal muscle and pancreas and, to a much lesser extent, in brain, liver and lung.

  • Pathway

    Purine metabolism; 3',5'-cyclic GMP degradation; GMP from 3',5'-cyclic GMP: step 1/1.

  • Sequence similarities

    Belongs to the cyclic nucleotide phosphodiesterase family.
    Contains 2 GAF domains.

  • Domain

    Composed of a C-terminal catalytic domain containing two putative divalent metal sites and an N-terminal regulatory domain which contains two homologous allosteric cGMP-binding regions, A and B.

  • Post-translational
    modifications

    Phosphorylation is regulated by binding of cGMP to the two allosteric sites.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • 5''-cyclic phosphodiesterase antibody
    • CGB PDE antibody
    • CGB-PDE antibody
    • CGBPDE antibody
    • cGMP binding cGMP specific 3' 5' cyclic nucleotide phosphodiesterase antibody
    • cGMP binding cGMP specific 35 cyclic nucleotide phosphodiesterase antibody
    • cGMP binding cGMP specific phosphodiesterase antibody
    • cGMP binding/cGMP specific phosphodiesterase antibody
    • cGMP specific 3'5' cyclic phosphodiesterase antibody
    • cGMP specific phosphodiesterase PDE5A2 antibody
    • cGMP specific phosphodiesterase type 5A antibody
    • cGMP-binding cGMP-specific phosphodiesterase antibody
    • cGMP-specific 3'' antibody
    • CN5A antibody
    • CN5N antibody
    • PDE 5 antibody
    • PDE 5A antibody
    • PDE5 antibody
    • Pde5a antibody
    • PDE5A_HUMAN antibody
    • PDE5A1 antibody
    • Phosphodiesterase 5A antibody
    • Phosphodiesterase 5A cGMP specific antibody
    • Phosphodiesterase isozyme 5 antibody
    see all

Images

  • Western blot - Anti-PDE5A/PDE5 antibody (ab28761)
    Western blot - Anti-PDE5A/PDE5 antibody (ab28761)
    Anti-PDE5A/PDE5 antibody (ab28761) at 1 µg/ml + lysate prepared from transfected HEK293 cells transiently expressing PDE5A/PDE5 (isoform 2)

    Predicted band size: 100 kDa
    Observed band size: 95 kDa
    why is the actual band size different from the predicted?

  • Flow Cytometry - Anti-PDE5A/PDE5 antibody (ab28761)
    Flow Cytometry - Anti-PDE5A/PDE5 antibody (ab28761)

    Flow cytometric analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation with ab28761 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (1 μg/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

  • Immunocytochemistry/ Immunofluorescence - Anti-PDE5A/PDE5 antibody (ab28761)
    Immunocytochemistry/ Immunofluorescence - Anti-PDE5A/PDE5 antibody (ab28761)

    Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation with ab28761 for 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).

References

ab28761 has not yet been referenced specifically in any publications.

Publishing research using ab28761? Please let us know so that we can cite the reference in this datasheet.

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Western blot abreview for Anti-PDE5A/PDE5 antibody

 Good
Abreviews
abreview image
Application
Western blot
Sample
Grey seal Tissue lysate - whole (Lung)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
20 µg
Specification
Lung
Blocking step
Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
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Submitted Mar 01 2017

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