Western blot abreview for Anti-PDE5A/PDE5 antibody

Good
Abreviews
Application
Western blot
Sample
Grey seal Tissue lysate - whole (Lung)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
20 µg
Specification
Lung
Blocking step
Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C

Other product details

Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Odyssey Blocking Buffer
Concentration
1 µg/ml

Secondary antibody

Dilution
1/10000
Name
Non-Abcam antibody was used: IRDye® 680RD Donkey anti-Goat IgG
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye® 680RD

Detection

Detection method
Infrared
Exposure
1 hour(s), 0 minute(s) and 0 second(s)
Bands
Specific: 100 kDa Non-specific: 40, 25 kDa
Positive control
mouse lung (third band)

Additional data

Additional Notes
Weddell seal lung rather than grey seal (Weddell seal was not an option in the menu). The samples were: two weddell seal lungs, mouse lung, sheep lung.

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Submitted Mar 01 2017

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