• Product name

    Anti-PDE6 beta/PDE6B antibody
  • Description

    Rabbit polyclonal to PDE6 beta/PDE6B
  • Host species

  • Tested applications

    Suitable for: IHC-P, IP, WB, ICC/IF, IHC-Frmore details
  • Species reactivity

    Reacts with: Mouse, Cow, Human
    Predicted to work with: Dog
  • Immunogen

    Synthetic peptide corresponding to Mouse PDE6 beta/PDE6B aa 20-36.


    (Peptide available as ab5890)

  • General notes

     This product was previously labelled as PDE6 beta



  • Form

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituents: 99% PBS, 0.1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Primary antibody notes

    The second messengers cAMP and cGMP are key regulatory molecules that are involved in a wide variety of signal transduction pathways, such as insulin secretion, platelet aggregation, smooth muscle relaxation, olfaction, and vision. Levels of cAMP and cGMP are regulated by their rate of synthesis by nucleotide cyclases and by their rate of hydrolysis by cyclic nucleotide phosphodiesterases (PDEs). PDEs form a superfamily of enzymes that catalyze the conversion of 3-prime, 5-prime-cyclic nucleotides to the corresponding nucleoside 5-prime-monophosphates. While mammalian PDEs are divided into major families based on their substrate specificities, kinetic properties, allosteric regulators, inhibitor sensitivities, and amino acid sequences, each family and even members within a family display distinct tissue, cell, and subcellular expression patters. This suggests that individual PDE family members are involved in discrete signal transduction pathways. PDE6 is the effector enzyme in the G protein-mediated signal transduction cascade in the visual system. There are five different subunits consisting of rod and cone specific catalytic subunits: alpha’ (Cone), alpha (Rod), and beta (Rod), the inhibitory subunit gamma, and subunit delta of unknown function (which likely interacts with many other proteins besides the PDE6 family). The catalytic core of the PDE6 system is comprised of alpha’/alpha’ homodimers in the cone and alpha/beta heterodimers in the rod. The C-terminus of both the catalytic and inhibitory subunits is modified by methylation, myristyolation and prenylation which have been shown to be critical for proper complex assembly and membrane association.
  • Clonality

  • Isotype

  • Research areas


Our Abpromise guarantee covers the use of ab5663 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use at an assay dependent concentration.
WB Use a concentration of 2 µg/ml. Detects a band of approximately 90 kDa.
ICC/IF Use at an assay dependent concentration. PubMed: 17960120
IHC-Fr Use at an assay dependent concentration.


  • Function

    This protein participates in processes of transmission and amplification of the visual signal. Necessary for the formation of a functional phosphodiesterase holoenzyme.
  • Involvement in disease

    Defects in PDE6B are the cause of retinitis pigmentosa type 40 (RP40) [MIM:613801]. RP40 is a retinal dystrophy belonging to the group of pigmentary retinopathies. Retinitis pigmentosa is characterized by retinal pigment deposits visible on fundus examination and primary loss of rod photoreceptor cells followed by secondary loss of cone photoreceptors. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well.
    Defects in PDE6B are a cause of congenital stationary night blindness autosomal dominant type 2 (CSNBAD2) [MIM:163500]; also known as congenital stationary night blindness Rambusch type. Congenital stationary night blindness is a non-progressive retinal disorder characterized by impaired night vision.
  • Sequence similarities

    Belongs to the cyclic nucleotide phosphodiesterase family.
    Contains 2 GAF domains.
  • Cellular localization

  • Information by UniProt
  • Database links

  • Alternative names

    • 5''-cyclic phosphodiesterase subunit beta antibody
    • Congenital stationary night blindness 3 autosomal dominant antibody
    • CSNB 3 antibody
    • CSNB3 antibody
    • CSNBAD2 antibody
    • GMP PDE beta antibody
    • GMP-PDE beta antibody
    • PDE 6 beta antibody
    • PDE 6B antibody
    • PDE6B antibody
    • PDE6B_HUMAN antibody
    • PDEB antibody
    • Phosphodiesterase 6B antibody
    • Phosphodiesterase 6B cGMP specific rod beta antibody
    • Rd 1 antibody
    • Rd1 antibody
    • Rod cGMP phosphodiesterase beta subunit antibody
    • Rod cGMP specific 3' 5' cyclic phosphodiesterase beta subunit antibody
    • Rod cGMP-specific 3'' antibody
    • RP40 antibody
    see all


  • Western blot of PDE6 beta/PDE6B using ab5663.
    Lane 1 demostrates no detection on extracts of RD mice.
    Lane 2 shows a single band at ~90 kDa on Balb/C extracts.

  • Immunofluorescences on frozen secions of mouse retina outer segments after staining with ab5663.

  • IHC image of ab5663 staining in mouse normal eye formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab5663, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Immunofluorescence analysis of murine retinal stem cells, staining PDE6 beta/PDE6B (green) with ab5663 at 1/100 dilution.


This product has been referenced in:

  • Yan W  et al. A Natural Occurring Mouse Model with Adgrv1 Mutation of Usher Syndrome 2C and Characterization of its Recombinant Inbred Strains. Cell Physiol Biochem 47:1883-1897 (2018). Read more (PubMed: 29961073) »
  • Sanges D  et al. Reprogramming Müller glia via in vivo cell fusion regenerates murine photoreceptors. J Clin Invest 126:3104-3116 (2016). WB ; Mouse . Read more (PubMed: 27427986) »
See all 8 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A


Thank you for contacting us and sorry for the delay in getting back to you.

Unfortunately I can confirm that the have not tested the Anti-PDE6 beta antibody (ab5663) with Rat samples. The image which was on the datasheet was unfortunately mislabeled. This has now been corrected. Thank you for bringing this to our attention.

Although ab5663 has not been tested with rat samples this does not mean that it would not work.Due to the high sequence homology of the immunogen used to raise the antibody with therat protein we would expect the antibody to cross react, however as we have not tested it we would not be able to guarantee this.

However, if you would like to purchase ab5663 and use it with yourrat samples, we can offer you a testing discount. This offer means that if you purchase ab5663 as normal, test the antibody with your rat samples and let us know of the results through an Abreview (no matter whether positive or negative) you would be eligible for a free primary antibody of your choice from our catalogue (or the equivalent price of ab5663 off any product). More information on this offer can be found here:


Please not that the antibody to be tested must be purchased, tested, the Abreview submitted and the free product claimed within a 4 month period.

If you would be interested in participating in this scheme please do let me know as a discount code needs to be issued prior to the purchase of ab5663.

I hope this information has been of help. If you require any further information please do not hesitate to contact us again.

Read More
Immunohistochemistry (Frozen sections)
Mouse Tissue sections (Skin, Retina)
Yes - 0.4% TritonX-100
Skin, Retina
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C

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