Overview

  • Product name

  • Description

    Rabbit polyclonal to PDE6D
  • Host species

    Rabbit
  • Specificity

    This antibody does not detect other PDE6 isoforms.
  • Tested applications

    Suitable for: ICC, ICC/IF, IHC-Fr, WBmore details
  • Species reactivity

    Reacts with: Mouse, Sheep, Cow, Dog, Human, Pig
  • Immunogen

    Synthetic peptide corresponding to Mouse PDE6D aa 1-16.
    Sequence:

    MSAKDERAR(D/E)ILRGFK


    (Peptide available as ab41788)

  • Positive control

    • WB: bovine retinal extracts.
  • General notes

     This product was previously labelled as PDE6 delta

     

Properties

Applications

Our Abpromise guarantee covers the use of ab5665 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC 1/10 - 1/200.
ICC/IF 1/10 - 1/200.
IHC-Fr Use a concentration of 1 µg/ml.

Immunohistochemical staining of PDE6D in bovine retinal cross-sections using ab5665 results in staining of retinal outer segments.

WB Use a concentration of 2 µg/ml. Detects a band of approximately 17 kDa.

Target

  • Function

    Acts as a GTP specific dissociation inhibitor (GDI). Increases the affinity of ARL3 for GTP by several orders of magnitude and does so by decreasing the nucleotide dissociation rate. Stabilizes Arl3-GTP by decreasing the nucleotide dissociation.
  • Tissue specificity

    Retina.
  • Sequence similarities

    Belongs to the PDE6D/unc-119 family.
  • Information by UniProt
  • Database links

  • Alternative names

    • 5''-cyclic phosphodiesterase subunit delta antibody
    • GMP PDE delta antibody
    • GMP-PDE delta antibody
    • p17 protein antibody
    • PDE 6 antibody
    • PDE 6D antibody
    • PDE D antibody
    • PDE6 antibody
    • PDE6D antibody
    • PDE6D_HUMAN antibody
    • PDED antibody
    • Phosphodiesterase 6D cGMP specific antibody
    • Phosphodiesterase 6D cGMP specific rod delta antibody
    • Protein p17 antibody
    • Retinal rod photoreceptor cGMP phosphodiesterase delta antibody
    • Retinal rod rhodopsin sensitive cGMP 3' 5' cyclic phosphodiesterase subunit delta antibody
    • Retinal rod rhodopsin-sensitive cGMP 3'' antibody
    see all

Images

  • Immunocytochemistry/Immunofluorescence analysis of PDE6D (green) showing staining in the cytoplasm and nucleus of B-3 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab5565 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

  • Immunocytochemistry/Immunofluorescence analysis of PDE6D (green) showing staining in the cytoplasm and nucleus of BAEC cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab5665 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

  • Ab5665 (1/750) staining PDE6D in MDCK cells
    Secondary antibody: An IRDye® Donkey anti-rabbit monoclonal (1/10000)
    Reduced denaturing conditions
    Blocking step: 10% Licor blocking buffer for 1 hour at 25°C

    See Abreview

References

This product has been referenced in:

  • Li G  et al. Generation of Retinal Organoids with Mature Rods and Cones from Urine-Derived Human Induced Pluripotent Stem Cells. Stem Cells Int 2018:4968658 (2018). Read more (PubMed: 30008752) »
See 1 Publication for this product

Customer reviews and Q&As

Application
Western blot
Sample
Dog Cell lysate - whole cell (Liver cells,MDCK)
Gel Running Conditions
Reduced Denaturing (4-20% Precise protein gels)
Loading amount
50 µg
Treatment
siRNA against PDE6D in different concentrations
Specification
Liver cells,MDCK
Blocking step
Licor blocking buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Nov 27 2009

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up