Overview

  • Product name

  • Description

    Rabbit polyclonal to PDE6D
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Dog, Zebrafish
  • Immunogen

    Recombinant fragment corresponding to Human PDE6D aa 1-120.
    Database link: NP_002592

  • Positive control

    • WB: MOLT4 whole cell lysate; mouse eye lysate. IHC-P: SAS xenograft tissue. ICC/IF: HeLa cells.
  • General notes

     This product was previously labelled as PDE6 delta

     

Properties

Applications

Our Abpromise guarantee covers the use of ab96825 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/1000.
IHC-P 1/100 - 1/1000.
WB 1/500 - 1/5000. Predicted molecular weight: 17 kDa.

Target

  • Function

    Acts as a GTP specific dissociation inhibitor (GDI). Increases the affinity of ARL3 for GTP by several orders of magnitude and does so by decreasing the nucleotide dissociation rate. Stabilizes Arl3-GTP by decreasing the nucleotide dissociation.
  • Tissue specificity

    Retina.
  • Sequence similarities

    Belongs to the PDE6D/unc-119 family.
  • Information by UniProt
  • Database links

  • Alternative names

    • 5''-cyclic phosphodiesterase subunit delta antibody
    • GMP PDE delta antibody
    • GMP-PDE delta antibody
    • p17 protein antibody
    • PDE 6 antibody
    • PDE 6D antibody
    • PDE D antibody
    • PDE6 antibody
    • PDE6D antibody
    • PDE6D_HUMAN antibody
    • PDED antibody
    • Phosphodiesterase 6D cGMP specific antibody
    • Phosphodiesterase 6D cGMP specific rod delta antibody
    • Protein p17 antibody
    • Retinal rod photoreceptor cGMP phosphodiesterase delta antibody
    • Retinal rod rhodopsin sensitive cGMP 3' 5' cyclic phosphodiesterase subunit delta antibody
    • Retinal rod rhodopsin-sensitive cGMP 3'' antibody
    see all

Images

  • Paraffin-embedded SAS xenograft tissue labeling PDE6D with ab96825 at 1/500 dilution in immunohistochemical analysis.

    Antigen retrieval: EDTA-based (pH 8.0), 15 minutes.

  • Methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling PDE6D with ab96825 at 1/500 dilution (green) in ICC/IF.

    The nuclear counter stain is Hoechst 33342 (blue).

  • Anti-PDE6D antibody (ab96825) at 1/5000 dilution + Mouse eye lysate at 50 µg

    Secondary
    Anti-Rabbit IgG HRP

    Predicted band size: 17 kDa

  • Anti-PDE6D antibody (ab96825) at 1/1000 dilution + MOLT4 whole cell lysate at 30 µg

    Predicted band size: 17 kDa



    12% SDS PAGE

References

This product has been referenced in:

  • Chen YH  et al. EPHA2 feedback activation limits the response to PDEd inhibition in KRAS-dependent cancer cells. Acta Pharmacol Sin N/A:N/A (2019). Read more (PubMed: 31316177) »
See 1 Publication for this product

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