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Rabbit monoclonal [EPR7731] to PDE7A
ICC/IF more details Unsuitable for:
Flow Cyt or IP
Synthetic peptide, corresponding to residues in Human PDE7A (Uniprot ID Q13946).
HuT78 cell lysate; Human skeletal muscle tissue; HuT78 cells.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab ® patents
This product is a recombinant rabbit monoclonal antibody.
Shipped at 4°C. Store at -20ºC.
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Tissue culture supernatant
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000. Predicted molecular weight: 56 kDa.
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
1/250 - 1/500.
Is unsuitable for Flow Cyt or IP.
Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May have a role in muscle signal transduction.
PDE7A1 is found at high levels in skeletal muscle and at low levels in a variety of tissues including brain and heart. It is expressed as well in two T-cell lines. PDE7A2 is found abundantly in skeletal muscle and at low levels in heart.
Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.
Belongs to the cyclic nucleotide phosphodiesterase family. PDE7 subfamily.
Developmentally regulated. PDE7A1 and PDE7A2 are found in several fetal tissues, expression is reduced throughout development. It persists strongly only in adult skeletal muscle.
Composed of a C-terminal catalytic domain containing two putative divalent metal sites and an N-terminal regulatory domain.
Cytoplasm > cytosol. PDE7A1 (57 kDa) is located mostly to soluble cellular fractions and Cytoplasm. PDE7A2 (50 kDa) is located to particulate cellular fractions.
Information by UniProt
5''-cyclic phosphodiesterase 7A antibody
HCP 1 antibody
Western blot - Anti-PDE7A antibody [EPR7731] (ab154857)
Anti-PDE7A antibody [EPR7731] (ab154857) at 1/1000 dilution + HuT78 cell lysate at 10 µg
Secondary Goat anti-rabbit HRP at 1/2000 dilution Predicted band size: 56 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDE7A antibody [EPR7731] (ab154857)
Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling PDE7A with ab154857 at 1/100 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-PDE7A antibody [EPR7731] (ab154857)
Immunofluorescent analysis of HuT78 cells labeling PDE7A with ab154857 at 1/250 dilution.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"