Recombinant Anti-PDE7A/HCP1 antibody [EPR7731] (ab154857)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7731] to PDE7A/HCP1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-PDE7A/HCP1 antibody [EPR7731]
See all PDE7A/HCP1 primary antibodies -
Description
Rabbit monoclonal [EPR7731] to PDE7A/HCP1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human PDE7A/HCP1.
Database link: Q13946 -
Positive control
- HuT78 cell lysate; Human skeletal muscle tissue; HuT78 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7731 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154857 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/10000. Predicted molecular weight: 56 kDa.
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ICC/IF |
1/250 - 1/500.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 56 kDa. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ICC/IF
1/250 - 1/500. |
Target
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Function
Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May have a role in muscle signal transduction. -
Tissue specificity
PDE7A1 is found at high levels in skeletal muscle and at low levels in a variety of tissues including brain and heart. It is expressed as well in two T-cell lines. PDE7A2 is found abundantly in skeletal muscle and at low levels in heart. -
Pathway
Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1. -
Sequence similarities
Belongs to the cyclic nucleotide phosphodiesterase family. PDE7 subfamily. -
Developmental stage
Developmentally regulated. PDE7A1 and PDE7A2 are found in several fetal tissues, expression is reduced throughout development. It persists strongly only in adult skeletal muscle. -
Domain
Composed of a C-terminal catalytic domain containing two putative divalent metal sites and an N-terminal regulatory domain. -
Cellular localization
Cytoplasm > cytosol. PDE7A1 (57 kDa) is located mostly to soluble cellular fractions and Cytoplasm. PDE7A2 (50 kDa) is located to particulate cellular fractions. - Information by UniProt
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Database links
- Entrez Gene: 5150 Human
- Omim: 171885 Human
- SwissProt: Q13946 Human
- Unigene: 527119 Human
- Unigene: 728847 Human
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Alternative names
- 5''-cyclic phosphodiesterase 7A antibody
- HCP 1 antibody
- HCP1 antibody
see all
Images
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Anti-PDE7A/HCP1 antibody [EPR7731] (ab154857) at 1/1000 dilution + HuT78 cell lysate at 10 µg
Secondary
Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 56 kDa -
Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling PDE7A/HCP1 with ab154857 at 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunofluorescent analysis of HuT78 cells labeling PDE7A/HCP1 with ab154857 at 1/250 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab154857 has been referenced in 3 publications.
- Cai Y et al. Genomic Alterations in PIK3CA-Mutated Breast Cancer Result in mTORC1 Activation and Limit the Sensitivity to PI3Kα Inhibitors. Cancer Res 81:2470-2480 (2021). PubMed: 33685991
- Li EA et al. Phosphodiesterase expression in the normal and failing heart. Arch Biochem Biophys 662:160-168 (2019). PubMed: 30550727
- Yamamoto N et al. The tumor-suppressive microRNA-1/133a cluster targets PDE7A and inhibits cancer cell migration and invasion in endometrial cancer. Int J Oncol 47:325-34 (2015). PubMed: 25955017