Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7731] to PDE7A/HCP1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Product nameAnti-PDE7A/HCP1 antibody [EPR7731]
See all PDE7A/HCP1 primary antibodies
DescriptionRabbit monoclonal [EPR7731] to PDE7A/HCP1
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt or IP
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human PDE7A/HCP1.
Database link: Q13946
- HuT78 cell lysate; Human skeletal muscle tissue; HuT78 cells.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product was previously labelled as PDE7A
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20ºC.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab154857 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 56 kDa.|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ICC/IF||1/250 - 1/500.|
FunctionHydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May have a role in muscle signal transduction.
Tissue specificityPDE7A1 is found at high levels in skeletal muscle and at low levels in a variety of tissues including brain and heart. It is expressed as well in two T-cell lines. PDE7A2 is found abundantly in skeletal muscle and at low levels in heart.
PathwayPurine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.
Sequence similaritiesBelongs to the cyclic nucleotide phosphodiesterase family. PDE7 subfamily.
Developmental stageDevelopmentally regulated. PDE7A1 and PDE7A2 are found in several fetal tissues, expression is reduced throughout development. It persists strongly only in adult skeletal muscle.
DomainComposed of a C-terminal catalytic domain containing two putative divalent metal sites and an N-terminal regulatory domain.
Cellular localizationCytoplasm > cytosol. PDE7A1 (57 kDa) is located mostly to soluble cellular fractions and Cytoplasm. PDE7A2 (50 kDa) is located to particulate cellular fractions.
- Information by UniProt
- 5''-cyclic phosphodiesterase 7A antibody
- HCP 1 antibody
- HCP1 antibody
Anti-PDE7A/HCP1 antibody [EPR7731] (ab154857) at 1/1000 dilution + HuT78 cell lysate at 10 µg
Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 56 kDa
Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling PDE7A/HCP1 with ab154857 at 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunofluorescent analysis of HuT78 cells labeling PDE7A/HCP1 with ab154857 at 1/250 dilution.
ab154857 has been referenced in 1 publication.
- Li EA et al. Phosphodiesterase expression in the normal and failing heart. Arch Biochem Biophys 662:160-168 (2019). PubMed: 30550727