Anti-PDE8A antibody (ab109597)
Key features and details
- Rabbit polyclonal to PDE8A
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PDE8A antibody
See all PDE8A primary antibodies -
Description
Rabbit polyclonal to PDE8A -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to a region between residue 779 and 829 of Human PDE8A (NP_002596.1).
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Positive control
- HeLa and 293T whole cell lysates.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab109597 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/2000 - 1/10000. Predicted molecular weight: 93 kDa. | |
| IP | Use at 2-5 µg/mg of lysate. |
Target
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Function
Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May be involved in maintaining basal levels of the cyclic nucleotide and/or in the cAMP regulation of germ cell development. -
Tissue specificity
Expressed in most tissues except thymus and peripheral blood leukocytes. Highest levels in testis, ovary, small intestine and colon. -
Pathway
Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1. -
Sequence similarities
Belongs to the cyclic nucleotide phosphodiesterase family. PDE8 subfamily.
Contains 1 PAC (PAS-associated C-terminal) domain.
Contains 1 PAS (PER-ARNT-SIM) domain. -
Domain
Composed of a C-terminal catalytic domain containing two putative divalent metal sites and an N-terminal regulatory domain. - Information by UniProt
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Database links
- Entrez Gene: 5151 Human
- Omim: 602972 Human
- SwissProt: O60658 Human
- Unigene: 9333 Human
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Alternative names
- 5''-cyclic phosphodiesterase 8A antibody
- cAMP specific cyclic nucleotide phosphodiesterase 8A antibody
- FLJ16150 antibody
see all
Images
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Western blot - Anti-PDE8A antibody (ab109597)All lanes : Anti-PDE8A antibody (ab109597) at 0.1 µg/ml
Lane 1 : HeLa cell lysate at 50 µg
Lane 2 : HeLa cell lysate at 15 µg
Lane 3 : HeLa cell lysate at 5 µg
Lane 4 : 293T cell lysate at 50 µg
Predicted band size: 93 kDa -
Immunoprecipitation - Anti-PDE8A antibody (ab109597)Detection of PDE8A by Western Blot of Immunprecipitate.
Lane 1: ab109597 at 1µg/ml staining PDE8A in HeLa whole cell lysate immunoprecipitated using ab109597 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Lane 2: Control IgG
Detection: Chemiluminescence with exposure time of 30 seconds.
All lanes : Anti-PDE8A antibody (ab109597) at 1 µg
Lane 1 : IP of 6 µg of PDE8A antibody/mg HeLa cell lysate
Lane 2 : Control IgG with HeLa cell lysate
Lysates/proteins at 1000 µg per lane.
Protocols
Datasheets and documents
References (0)
ab109597 has not yet been referenced specifically in any publications.
