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    pde8a-antibody-ab109597.pdf

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Signal Transduction Second Messenger Nucleotide Messenger cAMP
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Anti-PDE8A antibody (ab109597)

  • Datasheet
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Abpromise

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Western blot - Anti-PDE8A antibody (ab109597)
  • Immunoprecipitation - Anti-PDE8A antibody (ab109597)

Key features and details

  • Rabbit polyclonal to PDE8A
  • Suitable for: WB, IP
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Protein
Product image
Recombinant Human PDE8A protein (ab114643)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-PDE8A antibody
    See all PDE8A primary antibodies
  • Description

    Rabbit polyclonal to PDE8A
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to a region between residue 779 and 829 of Human PDE8A (NP_002596.1).

  • Positive control

    • HeLa and 293T whole cell lysates.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7
    Preservative: 0.09% Sodium azide
    Constituent: Tris citrate/phosphate
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Second Messenger
    • Nucleotide Messenger
    • cAMP

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab29545)
  • Recombinant Protein

    • Recombinant Human PDE8A protein (ab114643)

Applications

Our Abpromise guarantee covers the use of ab109597 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 93 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • Function

    Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May be involved in maintaining basal levels of the cyclic nucleotide and/or in the cAMP regulation of germ cell development.
  • Tissue specificity

    Expressed in most tissues except thymus and peripheral blood leukocytes. Highest levels in testis, ovary, small intestine and colon.
  • Pathway

    Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.
  • Sequence similarities

    Belongs to the cyclic nucleotide phosphodiesterase family. PDE8 subfamily.
    Contains 1 PAC (PAS-associated C-terminal) domain.
    Contains 1 PAS (PER-ARNT-SIM) domain.
  • Domain

    Composed of a C-terminal catalytic domain containing two putative divalent metal sites and an N-terminal regulatory domain.
  • Target information above from: UniProt accession O60658 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 5151 Human
    • Omim: 602972 Human
    • SwissProt: O60658 Human
    • Unigene: 9333 Human
    • Alternative names

      • 5''-cyclic phosphodiesterase 8A antibody
      • cAMP specific cyclic nucleotide phosphodiesterase 8A antibody
      • FLJ16150 antibody
      • High affinity cAMP specific and IBMX insensitive 3'5' cyclic phosphodiesterase 8A antibody
      • High affinity cAMP-specific and IBMX-insensitive 3'' antibody
      • HsT19550 antibody
      • PDE 8A antibody
      • PDE8A antibody
      • PDE8A_HUMAN antibody
      • Phosphodiesterase 8A antibody
      • Phosphodiesterase8A antibody
      • Weakly similar to 3'5' cyclic nucleotide phosphodiesterase antibody
      see all

    Images

    • Western blot - Anti-PDE8A antibody (ab109597)
      Western blot - Anti-PDE8A antibody (ab109597)
      All lanes : Anti-PDE8A antibody (ab109597) at 0.1 µg/ml

      Lane 1 : HeLa cell lysate at 50 µg
      Lane 2 : HeLa cell lysate at 15 µg
      Lane 3 : HeLa cell lysate at 5 µg
      Lane 4 : 293T cell lysate at 50 µg

      Predicted band size: 93 kDa

    • Immunoprecipitation - Anti-PDE8A antibody (ab109597)
      Immunoprecipitation - Anti-PDE8A antibody (ab109597)
      Detection of PDE8A by Western Blot of Immunprecipitate.
      Lane 1: ab109597 at 1µg/ml staining PDE8A in HeLa whole cell lysate immunoprecipitated using ab109597 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
      Lane 2: Control IgG
      Detection: Chemiluminescence with exposure time of 30 seconds.

      All lanes : Anti-PDE8A antibody (ab109597) at 1 µg

      Lane 1 : IP of 6 µg of PDE8A antibody/mg HeLa cell lysate
      Lane 2 : Control IgG with HeLa cell lysate

      Lysates/proteins at 1000 µg per lane.

    Protocols

    • Western blot protocols
    • Immunoprecipitation protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab109597? Please let us know so that we can cite the reference in this datasheet.

    ab109597 has not yet been referenced specifically in any publications.

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