Key features and details
- Rabbit polyclonal to PDE8A
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PDE8A antibody
See all PDE8A primary antibodies
DescriptionRabbit polyclonal to PDE8A
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to a region between residue 779 and 829 of Human PDE8A (NP_002596.1).
- HeLa and 293T whole cell lysates.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab109597 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 93 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionHydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May be involved in maintaining basal levels of the cyclic nucleotide and/or in the cAMP regulation of germ cell development.
Tissue specificityExpressed in most tissues except thymus and peripheral blood leukocytes. Highest levels in testis, ovary, small intestine and colon.
PathwayPurine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.
Sequence similaritiesBelongs to the cyclic nucleotide phosphodiesterase family. PDE8 subfamily.
Contains 1 PAC (PAS-associated C-terminal) domain.
Contains 1 PAS (PER-ARNT-SIM) domain.
DomainComposed of a C-terminal catalytic domain containing two putative divalent metal sites and an N-terminal regulatory domain.
- Information by UniProt
- 5''-cyclic phosphodiesterase 8A antibody
- cAMP specific cyclic nucleotide phosphodiesterase 8A antibody
- FLJ16150 antibody
All lanes : Anti-PDE8A antibody (ab109597) at 0.1 µg/ml
Lane 1 : HeLa cell lysate at 50 µg
Lane 2 : HeLa cell lysate at 15 µg
Lane 3 : HeLa cell lysate at 5 µg
Lane 4 : 293T cell lysate at 50 µg
Predicted band size: 93 kDa
Detection of PDE8A by Western Blot of Immunprecipitate.
Lane 1: ab109597 at 1µg/ml staining PDE8A in HeLa whole cell lysate immunoprecipitated using ab109597 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Lane 2: Control IgG
Detection: Chemiluminescence with exposure time of 30 seconds.
All lanes : Anti-PDE8A antibody (ab109597) at 1 µg
Lane 1 : IP of 6 µg of PDE8A antibody/mg HeLa cell lysate
Lane 2 : Control IgG with HeLa cell lysate
Lysates/proteins at 1000 µg per lane.
ab109597 has not yet been referenced specifically in any publications.