Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May be involved in specific signaling in the thyroid gland.
Abundantly expressed in the thyroid. Also very weakly expressed in brain, spinal cord and placenta. In the thyroid isoform 1 predominates, and isoforms 2 and 6 are also highly expressed. In the placenta isoforms 1 and 2 are expressed equally. In the brain isoform 2 predominates.
Defects in PDE8B are the cause of striatal degeneration autosomal dominant (ADSD) [MIM:609161]. It is a movement disorder affecting the striatal part of the basal ganglia and characterized by bradykinesia, dysarthria and muscle rigidity. These symptoms resemble idiopathic Parkinson disease, but tremor is not present.
Belongs to the cyclic nucleotide phosphodiesterase family. PDE8 subfamily. Contains 1 PAS (PER-ARNT-SIM) domain.
Composed of a C-terminal catalytic domain containing two putative divalent metal sites and an N-terminal regulatory domain.
Immunoprecipitation of PDE8B.
Samples: Whole cell lysate from HeLa cells.
20% of IP loaded.
Antibodies: Affinity purified rabbit anti-PDE8B antibody
(ab112024) at 1 µg/ml. Lane 1- PDE8B was immunoprecipitated by rabbit anti-PDE8B antibody (ab112024)
at 6 mcg/mg lysate. Lane 2- PDE8B was
immunoprecipitated by an rabbit anti-PDE8B antibody that recognizes an upstream epitope. Lane 3- Rabbit IgG control. Detection: Chemiluminescence with exposure times of 3 minutes.
Western blot - Anti-PDE8B antibody (ab112024)
All lanes : Anti-PDE8B antibody (ab112024) at 0.4 µg/ml
Lane 1 : Whole cell extracts from HeLa cells at 50 µg Lane 2 : Whole cell extracts from HeLa cells at 15 µg Lane 3 : Whole cell extracts from HeLa cells at 5 µg