Key features and details
- Rabbit polyclonal to PDE8B
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PDE8B antibody
See all PDE8B primary antibodies
DescriptionRabbit polyclonal to PDE8B
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide, corresponding to a region within C terminal amino acids 835-885 of Human PDE8B (NP_003710.1)
- Whole cell lysate from HeLa cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab112024 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 99 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionHydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May be involved in specific signaling in the thyroid gland.
Tissue specificityAbundantly expressed in the thyroid. Also very weakly expressed in brain, spinal cord and placenta. In the thyroid isoform 1 predominates, and isoforms 2 and 6 are also highly expressed. In the placenta isoforms 1 and 2 are expressed equally. In the brain isoform 2 predominates.
PathwayPurine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.
Involvement in diseaseDefects in PDE8B are the cause of striatal degeneration autosomal dominant (ADSD) [MIM:609161]. It is a movement disorder affecting the striatal part of the basal ganglia and characterized by bradykinesia, dysarthria and muscle rigidity. These symptoms resemble idiopathic Parkinson disease, but tremor is not present.
Sequence similaritiesBelongs to the cyclic nucleotide phosphodiesterase family. PDE8 subfamily.
Contains 1 PAS (PER-ARNT-SIM) domain.
DomainComposed of a C-terminal catalytic domain containing two putative divalent metal sites and an N-terminal regulatory domain.
- Information by UniProt
- 3' 5' cyclic nucleotide phosphodiesterase 8B antibody
- 3'5' cyclic nucleotide phosphodiesterase 8B antibody
- Cell proliferation-inducing gene 22 protein antibody
Immunoprecipitation of PDE8B. Samples: Whole cell lysate from HeLa cells. 20% of IP loaded. Antibodies: Affinity purified rabbit anti-PDE8B antibody (ab112024) at 1 µg/ml. Lane 1- PDE8B was immunoprecipitated by rabbit anti-PDE8B antibody (ab112024) at 6 mcg/mg lysate. Lane 2- PDE8B was immunoprecipitated by an rabbit anti-PDE8B antibody that recognizes an upstream epitope. Lane 3- Rabbit IgG control. Detection: Chemiluminescence with exposure times of 3 minutes.
All lanes : Anti-PDE8B antibody (ab112024) at 0.4 µg/ml
Lane 1 : Whole cell extracts from HeLa cells at 50 µg
Lane 2 : Whole cell extracts from HeLa cells at 15 µg
Lane 3 : Whole cell extracts from HeLa cells at 5 µg
Predicted band size: 99 kDa
ab112024 has not yet been referenced specifically in any publications.