Anti-PDGF AA antibody (ab216619)
Key features and details
- Rabbit polyclonal to PDGF AA
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-PDGF AA antibody
See all PDGF AA primary antibodies -
Description
Rabbit polyclonal to PDGF AA -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide within Human PDGF AA aa 100-200 conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P04085 -
Positive control
- Human colon tubular adenocarcinoma tissue; Mouse lung and placenta lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 48.98% TBS, 1X -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab216619 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | (1) |
1/100 - 1/500.
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WB |
1/100 - 1/1000. Predicted molecular weight: 24 kDa.
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Notes |
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IHC-P
1/100 - 1/500. |
WB
1/100 - 1/1000. Predicted molecular weight: 24 kDa. |
Target
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Function
Platelet-derived growth factor is a potent mitogen for cells of mesenchymal origin. Binding of this growth factor to its receptor elicits a variety of cellular responses. It is released by platelets upon wounding and plays an important role in stimulating adjacent cells to grow and thereby heals the wound. -
Sequence similarities
Belongs to the PDGF/VEGF growth factor family. -
Domain
The long form contains a basic insert which acts as a cell retention signal. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 5154 Human
- Entrez Gene: 18590 Mouse
- Entrez Gene: 25266 Rat
- Omim: 173430 Human
- SwissProt: P04085 Human
- SwissProt: P20033 Mouse
- SwissProt: P28576 Rat
- Unigene: 535898 Human
see all -
Alternative names
- PDGF A antibody
- PDGF A chain antibody
- PDGF subunit A antibody
see all
Images
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded human colon tubular adenocarcinoma labeling PDGF AA with ab216619 at 1/300 dilution, followed by conjugation to the secondary antibody and DAB staining.
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Anti-PDGF AA antibody (ab216619) at 1/300 dilution + Mouse lung lysate
Secondary
Goat anti-rabbit IgG (H+L), HRP conjugated at 1/5000 dilution
Predicted band size: 24 kDa -
Anti-PDGF AA antibody (ab216619) at 1/300 dilution + Mouse placenta lysate
Secondary
Goat anti-rabbit IgG (H+L), HRP conjugated at 1/5000 dilution
Predicted band size: 24 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab216619 has been referenced in 3 publications.
- Tashjian RZ et al. The effect of estrogen-like compound on rotator cuff tendon healing in a murine model. J Orthop Res N/A:N/A (2021). PubMed: 33533088
- Yang F et al. Bone marrow mesenchymal stem cells induce M2 microglia polarization through PDGF-AA/MANF signaling. World J Stem Cells 12:633-658 (2020). PubMed: 32843919
- Yao Z et al. Reduced PDGF-AA in subchondral bone leads to articular cartilage degeneration after strenuous running. J Cell Physiol N/A:N/A (2019). PubMed: 30834523