Overview

  • Product name

  • Description

    Chicken Polyclonal to PDGF B
  • Host species

    Chicken
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human PDGF B aa 100-200 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    (Peptide available as ab140136)

  • Positive control

    • Ab139753 gave a positive signal when tested against PDGF BB recombinant proteins. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal kidney.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab139753 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use at an assay dependent concentration.

This antibody has been tested in WB against purified recombinant protein only. Abcam does not have data on how this antibody performs in WB when using tissue/ cell line lysates.

Target

  • Information by UniProt
  • Database links

  • Alternative names

    • Becaplermin antibody
    • c sis antibody
    • FLJ12858 antibody
    • Oncogene SIS antibody
    • PDGF 2 antibody
    • PDGF B chain antibody
    • PDGF Beta antibody
    • PDGF subunit B antibody
    • PDGF-2 antibody
    • PDGF2 antibody
    • Pdgfb antibody
    • PDGFB_HUMAN antibody
    • Platelet derived growth factor 2 antibody
    • Platelet derived growth factor B chain antibody
    • Platelet derived growth factor beta antibody
    • Platelet derived growth factor beta polypeptide (oncogene SIS) antibody
    • Platelet derived growth factor beta polypeptide (simian sarcoma viral (v sis) oncogene homolog) antibody
    • Platelet derived growth factor beta polypeptide antibody
    • Platelet-derived growth factor B chain antibody
    • Platelet-derived growth factor beta polypeptide antibody
    • Platelet-derived growth factor subunit B antibody
    • Proto-oncogene c-Sis antibody
    • Simian sarcoma viral (v sis) oncogene homolog antibody
    • SIS antibody
    • SSV antibody
    • v sis platelet derived growth factor beta polypeptide antibody
    see all

Images

  • All lanes : Anti-PDGF B antibody (ab139753) at 1 µg/ml

    Lane 1 : Recombinant Human PDGF B protein (ab73231)
    Lane 2 : Recombinant human PDGF BB protein (ab9706)

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Chicken IgY H&L (HRP) (ab6877) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Observed band size: 16.7 kDa
    why is the actual band size different from the predicted?


    Exposure time: 8 minutes


    ab73231 has a His tag so shows a high MW. 

  • IHC image of PDGF BB staining in Human normal kidney formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab139753, 1µg/ml, for 15 mins at room temperature. A Goat anti-Chicken biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References

ab139753 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab139753.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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