Anti-PDGF B antibody (ab23914)
- Datasheet
- References (9)
- Protocols
Overview
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Product nameAnti-PDGF B antibody
See all PDGF B primary antibodies -
DescriptionRabbit polyclonal to PDGF B
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Host speciesRabbit
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SpecificityThis antibody has been tested against PDGF BB protein (ab9706) and has yet to be tested against endogenous protein.
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Tested applicationsSuitable for: IHC-Fr, WB, IHC-Pmore details
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Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Cow
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human PDGF BB.
Read Abcam's proprietary immunogen policy (Peptide available as ab24035.) -
Positive control
- PDGF BB protein (ab9706)
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab23914 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr | Use at an assay dependent concentration. | |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 14 kDa (predicted molecular weight: 14-18 kDa). The predicted molecular weight of PDGF BB is ~ 27 kDa according to the Swissprot entry P01127, however the reduced form of PDGF BB runs between 14-18 kDa on a gel. |
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| IHC-P | Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
- Information by UniProt
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Database links
- Entrez Gene: 540106 Cow
- Entrez Gene: 5155 Human
- Entrez Gene: 18591 Mouse
- Entrez Gene: 24628 Rat
- Omim: 190040 Human
- SwissProt: P01127 Human
- SwissProt: P31240 Mouse
- SwissProt: Q05028 Rat
see all -
Alternative names
- Becaplermin antibody
- c sis antibody
- FLJ12858 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGF BB antibody (ab23914)This image is courtesy of an Abreview submitted by Lauren Carpenterab23914 staining PDGF BB (green) in mouse skeletal muscle tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 10% serum for 10 minutes at 25°C; antigen retrieval was by heat mediation in Tris pH 9. Samples were incubated with primary antibody (1/500 in antibody diluent reagent) for 14 hours at 4°C. An Alexa Fluor® 647-conjugated goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody. DAPI (blue) - nuclei.
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Western blot - Anti-PDGF BB antibody (ab23914)All lanes : Anti-PDGF B antibody (ab23914) at 1 µg/ml
Lane 1 :Recombinant human PDGF B protein (ab9706)
Lane 2 :Recombinant human PDGF B protein (ab9706) with Human PDGF B peptide (ab24035) at 1 µg
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 14-18 kDa
Observed band size: 14 kDa why is the actual band size different from the predicted?ab23914 detects a band of ~ 14 kDa corresponding to the reduced form of recombinant PDGF BB (ab9706). The predicted molecular weight for PDGF BB is 27 kDa according to the Swissprot entry, however the reduced form is known to run at between 14-18 kDa. The 14 kDa band is specifically blocked by the addition of the immunizing peptide, ab 24035.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGF BB antibody (ab23914)Image courtesy of Human Protein Atlas
ab23914 staining PDGF BB in Human kidney (male). The paraffin embedded tissue was incubated with ab23914 (1/500 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6. ab23914 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray con
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGF BB antibody (ab23914)This image is courtesy of an anonymous Abreviewab23914 staining PDGF BB (green) in Mouse skeletal muscle (gastrocnemius) tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with methacarn and blocked with 10% serum for 10 minutes at 25°C; antigen retrieval was by heat mediation in Tris pH 8. Samples were incubated with primary antibody (1/250 in antibody diluent reagent) for 14 hours at 4°C. An Alexa Fluor® 488-conjugated Goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody. Red - WGA membranes, Blue - DAPI Nuclei.
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Western blot - Anti-PDGF BB antibody (ab23914) -
Immunohistochemistry (Frozen sections) - Anti-PDGF BB antibody (ab23914)This image is courtesy of an anonymous Abreviewab23914 staining PDGF BB in Human skeletal muscle (gastrocnemius) tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with formaldehyde and blocked with 10% serum for 20 minutes at 25°C. Samples were incubated with primary antibody (1/150) for 14 hours at 4°C. An Alexa Fluor® 555-conjugated Goat anti-rabbit IgG polyclonal (1/5000) was used as the secondary antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGF BB antibody (ab23914)IHC image of PDGF BB staining in human heart formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab23914, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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Western blot
References
This product has been referenced in:
- Ono Y et al. CD11c+ M1-like macrophages (MFs) but not CD206+ M2-like MF are involved in folliculogenesis in mice ovary. Sci Rep 8:8171 (2018). Read more (PubMed: 29802255) »
- Shi R et al. Nanosphere-mediated co-delivery of VEGF-A and PDGF-B genes for accelerating diabetic foot ulcers healing in rats. Gene Ther 25:425-438 (2018). Read more (PubMed: 29955127) »
