Anti-PDGF B antibody (ab23914)
Rabbit polyclonal PDGF B antibody. Validated in WB, IHC and tested in Mouse, Human. Cited in 15 publication(s). Independently reviewed in 9 review(s). Immunogen corresponding to synthetic peptide.
- Datasheet
- References (23)
- Protocols
Overview
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Product name
Anti-PDGF B antibody
See all PDGF B primary antibodies -
Description
Rabbit polyclonal to PDGF B -
Host species
Rabbit -
Specificity
This antibody has been tested against PDGF BB protein (ab9706) and has yet to be tested against endogenous protein. -
Tested applications
Suitable for: IHC-Fr, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow
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Immunogen
Synthetic peptide corresponding to Human PDGF B aa 100-200 conjugated to keyhole limpet haemocyanin.
(Peptide available asab24035) -
Positive control
- PDGF BB protein (ab9706)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab23914 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr | Use at an assay dependent concentration. | |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 14 kDa (predicted molecular weight: 14-18 kDa). The predicted molecular weight of PDGF BB is ~ 27 kDa according to the Swissprot entry P01127, however the reduced form of PDGF BB runs between 14-18 kDa on a gel. |
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| IHC-P | Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
- Information by UniProt
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Database links
- Entrez Gene: 540106 Cow
- Entrez Gene: 5155 Human
- Entrez Gene: 18591 Mouse
- Entrez Gene: 24628 Rat
- Omim: 190040 Human
- SwissProt: P01127 Human
- SwissProt: P31240 Mouse
- SwissProt: Q05028 Rat
see all -
Alternative names
- Becaplermin antibody
- c sis antibody
- FLJ12858 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGF BB antibody (ab23914)This image is courtesy of an Abreview submitted by Lauren Carpenterab23914 staining PDGF BB (green) in mouse skeletal muscle tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 10% serum for 10 minutes at 25°C; antigen retrieval was by heat mediation in Tris pH 9. Samples were incubated with primary antibody (1/500 in antibody diluent reagent) for 14 hours at 4°C. An Alexa Fluor® 647-conjugated goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody. DAPI (blue) - nuclei.
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Western blot - Anti-PDGF BB antibody (ab23914)All lanes : Anti-PDGF B antibody (ab23914) at 1 µg/ml
Lane 1 :Recombinant human PDGF BB protein (ab9706)
Lane 2 :Recombinant human PDGF BB protein (ab9706) with Human PDGF B peptide (ab24035) at 1 µg
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 14-18 kDa
Observed band size: 14 kDa why is the actual band size different from the predicted?ab23914 detects a band of ~ 14 kDa corresponding to the reduced form of recombinant PDGF BB (ab9706). The predicted molecular weight for PDGF BB is 27 kDa according to the Swissprot entry, however the reduced form is known to run at between 14-18 kDa. The 14 kDa band is specifically blocked by the addition of the immunizing peptide, ab 24035.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGF BB antibody (ab23914)Image courtesy of Human Protein Atlas
ab23914 staining PDGF BB in Human kidney (male). The paraffin embedded tissue was incubated with ab23914 (1/500 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6. ab23914 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray con
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGF BB antibody (ab23914)This image is courtesy of an anonymous Abreviewab23914 staining PDGF BB (green) in Mouse skeletal muscle (gastrocnemius) tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with methacarn and blocked with 10% serum for 10 minutes at 25°C; antigen retrieval was by heat mediation in Tris pH 8. Samples were incubated with primary antibody (1/250 in antibody diluent reagent) for 14 hours at 4°C. An Alexa Fluor® 488-conjugated Goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody. Red - WGA membranes, Blue - DAPI Nuclei.
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Western blot - Anti-PDGF BB antibody (ab23914)Anti-PDGF B antibody (ab23914) at 1 µg/ml +Recombinant Human PDGF B protein (ab73231) at 0.01 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 14-18 kDa
Exposure time: 1 minute -
Immunohistochemistry (Frozen sections) - Anti-PDGF BB antibody (ab23914)This image is courtesy of an anonymous Abreviewab23914 staining PDGF BB in Human skeletal muscle (gastrocnemius) tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with formaldehyde and blocked with 10% serum for 20 minutes at 25°C. Samples were incubated with primary antibody (1/150) for 14 hours at 4°C. An Alexa Fluor® 555-conjugated Goat anti-rabbit IgG polyclonal (1/5000) was used as the secondary antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGF BB antibody (ab23914)IHC image of PDGF BB staining in human heart formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab23914, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
References
This product has been referenced in:
- Wang L et al. Mesenchymal Stem Cells Coated by the Extracellular Matrix Promote Wound Healing in Diabetic Rats. Stem Cells Int 2019:9564869 (2019). Read more (PubMed: 30833970) »
- Alhabibi AM et al. Platelet-derived growth factor-beta as a new marker of deep venous thrombosis. J Res Med Sci 24:48 (2019). Read more (PubMed: 31160915) »
