Product nameAnti-PDGF B antibody
See all PDGF B primary antibodies
DescriptionRabbit polyclonal to PDGF BB
Tested applicationsSuitable for: WB, ELISA, Neutralising, Sandwich ELISA, IHC-Pmore details
Species reactivityReacts with: Human
Highly pure (>98%) recombinant hPDGF-BB (human Platelet Derived Growth Factor)
- Recombinant human PDGF B protein (ab9706) can be used as a positive control in WB.
FormLyophilised:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferPBS, pH 7.4, no preservative, sterile filtered
Concentration information loading...
PurityImmunogen affinity purified
Light chain typeunknown
Our Abpromise guarantee covers the use of ab9704 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. To detect hPDGF-BB by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPDGF-BB is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.|
|ELISA||Use at an assay dependent concentration. To detect hPDGF-BB by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hPDGF-BB.|
|Neutralising||Use at an assay dependent concentration. To yield one-half maximal inhibition [ND50] of the biological activity of hPDGF-BB (2.5 ng/ml), a concentration of 0.03 - 0.04 µg/ml of this antibody is required.|
|Sandwich ELISA||Use at an assay dependent concentration. To be used with ab84265 as detection antibody and ab9706 as protein standard.|
|IHC-P||Use a concentration of 1 µg/ml.|
- Information by UniProt
- Becaplermin antibody
- c sis antibody
- FLJ12858 antibody
ab9704 staining PDGF BB in normal human skin tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent proteinase K mediated antigen retrieval. The primary antibody was used at 1 ug/ml and incubated with sample at 4°C overnight. A HRP-labeled polymer detection system was used with a non-alcohol soluble AEC chromogen.
Anti-PDGF B antibody (ab9704) at 0.2 µg/ml + A431 (Human) Whole Cell Lysate (ab52255) at 10 µg
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Observed band size: 18,29 kDa why is the actual band size different from the predicted?
Additional bands at: 45 kDa (possible dimer)
The 29kDa form is the propeptide.
This product has been referenced in:
- Zhao C et al. Endothelial Cords Promote Tumor Initial Growth prior to Vascular Function through a Paracrine Mechanism. Sci Rep 6:19404 (2016). Read more (PubMed: 26762853) »
- Yang J et al. Platelet-derived growth factor mediates survival of leukemic large granular lymphocytes via an autocrine regulatory pathway. Blood 115:51-60 (2010). Neutralising ; Human . Read more (PubMed: 19880494) »