Overview

  • Product name
    Anti-PDGF B antibody [EPR6834]
    See all PDGF B primary antibodies
  • Description
    Rabbit monoclonal [EPR6834] to PDGF B
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Sheep
  • Immunogen

    Synthetic peptide within Human PDGF B aa 200 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: P01127

  • Positive control
    • WB: Fetal brain, A375 and HeLa lysates. IHC-P: Human brain, heart and placenta tissues.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab178409 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.
  • Target

    • Information by UniProt
    • Database links
    • Alternative names
      • Becaplermin antibody
      • c sis antibody
      • FLJ12858 antibody
      • Oncogene SIS antibody
      • PDGF 2 antibody
      • PDGF B chain antibody
      • PDGF Beta antibody
      • PDGF subunit B antibody
      • PDGF-2 antibody
      • PDGF2 antibody
      • Pdgfb antibody
      • PDGFB_HUMAN antibody
      • Platelet derived growth factor 2 antibody
      • Platelet derived growth factor B chain antibody
      • Platelet derived growth factor beta antibody
      • Platelet derived growth factor beta polypeptide (oncogene SIS) antibody
      • Platelet derived growth factor beta polypeptide (simian sarcoma viral (v sis) oncogene homolog) antibody
      • Platelet derived growth factor beta polypeptide antibody
      • Platelet-derived growth factor B chain antibody
      • Platelet-derived growth factor beta polypeptide antibody
      • Platelet-derived growth factor subunit B antibody
      • Proto-oncogene c-Sis antibody
      • Simian sarcoma viral (v sis) oncogene homolog antibody
      • SIS antibody
      • SSV antibody
      • v sis platelet derived growth factor beta polypeptide antibody
      see all

    Images

    • All lanes : Anti-PDGF B antibody [EPR6834] (ab178409) at 1/1000 dilution

      Lane 1 : Fetal brain lysate
      Lane 2 : A375 lysate
      Lane 3 : HeLa lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 27 kDa

    • Immunohistochemical analysis of paraffin embedded Human placenta tissue labeling PDGF B with ab178409 at 1/50.

    • Immunohistochemical analysis of paraffin embedded Human heart tissue labeling PDGF B with ab178409 at 1/50.

    • Immunohistochemical analysis of paraffin embedded Human brain tissue labeling PDGF B with ab178409 at 1/50.

    References

    This product has been referenced in:
    • Liu F  et al. Quantitative proteomic analysis of gastric cancer tissue reveals novel proteins in platelet-derived growth factor b signaling pathway. Oncotarget 8:22059-22075 (2017). Read more (PubMed: 28423550) »
    • Wang H  et al. Growth of MCF-7 breast cancer cells and efficacy of anti-angiogenic agents in a hydroxyethyl chitosan/glycidyl methacrylate hydrogel. Cancer Cell Int 17:55 (2017). Read more (PubMed: 28515673) »

    See all 3 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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