Product nameAnti-PDGF B antibody [MM0014-5F66]
See all PDGF B primary antibodies
DescriptionMouse monoclonal [MM0014-5F66] to PDGF B
Tested applicationsSuitable for: ICC/IF, Flow Cyt, IP, IHC-P, WBmore details
Species reactivityReacts with: Human
Recombinant full length protein (Human)
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab51869 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration. PubMed: 24512807|
|Flow Cyt||Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|IHC-P||1/50 - 1/200.|
|WB||1/100 - 1/200. Predicted molecular weight: 27 kDa.|
- Information by UniProt
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ab51869 at 1/200 dilution, staining PDGF BB in human placental tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections).
Overlay histogram showing SHSY-5Y cells stained with ab51869 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab51869, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SHSY-5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
This product has been referenced in:
- Silva CR et al. Injectable and tunable hyaluronic acid hydrogels releasing chemotactic and angiogenic growth factors for endodontic regeneration. Acta Biomater 77:155-171 (2018). Read more (PubMed: 30031163) »
- Miyata T et al. The roles of platelet-derived growth factors and their receptors in brain radiation necrosis. Radiat Oncol 9:51 (2014). IHC-P, ICC/IF ; Human . Read more (PubMed: 24512807) »