Overview

  • Product name
    Anti-PDGF B antibody [MM0014-5F66]
    See all PDGF B primary antibodies
  • Description
    Mouse monoclonal [MM0014-5F66] to PDGF B
  • Host species
    Mouse
  • Tested applications
    Suitable for: ICC/IF, Flow Cyt, IP, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein (Human)

Properties

Applications

Our Abpromise guarantee covers the use of ab51869 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 24512807
Flow Cyt Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IP 1/50.
IHC-P 1/50 - 1/200.
WB 1/100 - 1/200. Predicted molecular weight: 27 kDa.

Target

  • Information by UniProt
  • Database links
  • Alternative names
    • Becaplermin antibody
    • c sis antibody
    • FLJ12858 antibody
    • Oncogene SIS antibody
    • PDGF 2 antibody
    • PDGF B chain antibody
    • PDGF Beta antibody
    • PDGF subunit B antibody
    • PDGF-2 antibody
    • PDGF2 antibody
    • Pdgfb antibody
    • PDGFB_HUMAN antibody
    • Platelet derived growth factor 2 antibody
    • Platelet derived growth factor B chain antibody
    • Platelet derived growth factor beta antibody
    • Platelet derived growth factor beta polypeptide (oncogene SIS) antibody
    • Platelet derived growth factor beta polypeptide (simian sarcoma viral (v sis) oncogene homolog) antibody
    • Platelet derived growth factor beta polypeptide antibody
    • Platelet-derived growth factor B chain antibody
    • Platelet-derived growth factor beta polypeptide antibody
    • Platelet-derived growth factor subunit B antibody
    • Proto-oncogene c-Sis antibody
    • Simian sarcoma viral (v sis) oncogene homolog antibody
    • SIS antibody
    • SSV antibody
    • v sis platelet derived growth factor beta polypeptide antibody
    see all

Images

  • ab51869 at 1/200 dilution, staining PDGF BB in human placental tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections).

  • Overlay histogram showing SHSY-5Y cells stained with ab51869 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab51869, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SHSY-5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

References

This product has been referenced in:
  • Silva CR  et al. Injectable and tunable hyaluronic acid hydrogels releasing chemotactic and angiogenic growth factors for endodontic regeneration. Acta Biomater 77:155-171 (2018). Read more (PubMed: 30031163) »
  • Miyata T  et al. The roles of platelet-derived growth factors and their receptors in brain radiation necrosis. Radiat Oncol 9:51 (2014). IHC-P, ICC/IF ; Human . Read more (PubMed: 24512807) »
See all 4 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (3T3)
Total protein in input
5e+007 cells
Specification
3T3
Immuno-precipitation step
Protein G

Abcam user community

Verified customer

Submitted Feb 05 2013

Answer

Thank you for contacting us, I apologize for the delay in my reply. I would recommend ab114130 for your purposes. The immunogen for this antibody shows no significant similarity to PDGFA, and it is slightly less likely to react with mouse than our other rabbit polyclonal antibodies. That being said, the mouse protein is fairly homologous with human and there is still a chance that ab114130 may show some reactivity to mouse PGDF BB.
If you do want to use the mouse monoclonal antibody ab51869 on mouse tissue, you may need to perform an additional blocking step to prevent mouse-on-mouse background staining. If mouse-on-mouse background is a problem in your assay, it will be visible when running your no primary antibody or isotype control. To reduce this background, you can perform an additional blocking step after your normal serum block and before the primary antibody incubation. The sections should be incubated for 1 hour with a F(ab) fragment unconjugated secondary antibody to mouse IgG, such as ab6668, at a ˜1:100 dilution at room temperature.
I hope this helps, please let me know if you need any additional information or assistance.

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