Product nameAnti-PDGF BB antibody
See all PDGF B primary antibodies
DescriptionRabbit polyclonal to PDGF BB
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse, Sheep, Cat, Dog
- Purchase matching WB positive control:Recombinant Human PDGF BB protein
- Recombinant human PDGFAA + PDGFBB protein (ab61903) can be used as a positive control in WB. Mesenchymal cells.
Storage instructionsShipped at 4°C. Store at 4°C (stable for up to 12 months).
Storage bufferPreservative: 0.1% Sodium azide
Constituents: 0.2% Gelatin, 0.82% Sodium phosphate
Concentration information loading...
Our Abpromise guarantee covers the use of ab16829 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 - 5 µg/ml.|
|WB||Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 27 kDa.|
|IHC-P||Use at an assay dependent concentration.|
- Information by UniProt
- Becaplermin antibody
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ab16829 staining PDGF BB (green) in mouse adipose tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 10% serum for 10 minutes at 25°C; antigen retrieval was enzymatic in Tris (pH 10). Samples were incubated with primary antibody (1/100 in anitbody diluent reagent) for 14 hours at 4°C. An Alexa Fluor® 647-conjugated goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody. Dlue - DAPI, Red - Elastin.
This product has been referenced in:
- Chen NF et al. Therapeutic Effect of Platelet-Rich Plasma in Rat Spinal Cord Injuries. Front Neurosci 12:252 (2018). Read more (PubMed: 29740270) »
- Chillo O et al. Perivascular Mast Cells Govern Shear Stress-Induced Arteriogenesis by Orchestrating Leukocyte Function. Cell Rep 16:2197-2207 (2016). Read more (PubMed: 27524614) »