The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/250 - 1/500. Detects a band of approximately 150,170 kDa (predicted molecular weight: 122 kDa).
Use at an assay dependent concentration. 5-10 µl immunoprecipitates PDGF Receptor alpha from 500 µg of NIH/3T3 RIPA lysate.
Receptor that binds both PDGFA and PDGFB and has a tyrosine-protein kinase activity.
Expressed in primary and metastatic colon tumors and in normal colon tissue. Tumors may express a different isoform to that found in normal tissue.
Involvement in disease
Note=A chromosomal aberration involving PDGFRA is found in some cases of hypereosinophilic syndrome. Interstitial chromosomal deletion del(4)(q12q12) causes the fusion of FIP1L1 and PDGFRA (FIP1L1-PDGFRA).
Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily. Contains 5 Ig-like C2-type (immunoglobulin-like) domains. Contains 1 protein kinase domain.
Stammnitz MR et al. The Origins and Vulnerabilities of Two Transmissible Cancers in Tasmanian Devils. Cancer Cell33:607-619.e15 (2018).
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Steger CM et al. Gene therapy with antisense oligonucleotides silencing c-myc reduces neointima formation and vessel wall thickness in a mouse model of vein graft disease. Exp Mol Pathol105:1-9 (2018).
Read more (PubMed: 29775572) »