Key features and details
- Rabbit polyclonal to PDGFR alpha + Beta (phospho Y572 + Y574)
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Product nameAnti-PDGFR alpha + Beta (phospho Y572 + Y574) antibody
DescriptionRabbit polyclonal to PDGFR alpha + Beta (phospho Y572 + Y574)
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse
Predicted to work with: Rat, Human, Xenopus laevis
Synthetic peptide corresponding to Human PDGFR alpha + Beta aa 572-574. This corresponds to Tyrosine 579 and 581 of Human PDGFR beta. The sequence is conserved in PDGFR alpha mouse, rat and frog.
- NIH3T3 cells +/- PDGF.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.1% BSA
BSA is IgG and protease free
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThe antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated PDGFR alpha / beta. The final product is generated by affinity chromatography using a PDGFR alpha derived peptide phosphorylated at Tyrosines 572 and 574.
Our Abpromise guarantee covers the use of ab5443 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.35 - 1 µg/ml. Detects a band of approximately 170 - 185 kDa.|
RelevancePlatelet derived growth factor receptor (PDGFR) is a transmembrane glycoprotein of 170-185 kDa which undergoes homo- or heterodimerization into complexes of alpha and beta subunits upon ligand binding, depending on the isoform of PDGF (PDGF-AA, -BB or -AB) that binds. The phosphorylation of tyrosine residues in the now activated receptor can control multiple signaling events such as actin reorganization, transcription, cell growth, migration and differentiation. PDGFR alpha tyrosines 572 and 574 (579 and 581 in PDGFR beta) are autophosphorylated in the activated receptor, and bind and activate Src family kinases.
Cellular localizationMembrane; single pass type I membrane protein.
- CD140A antibody
- CD140a antigen antibody
- CD140b antibody
Peptide Competition: Extracts prepared from NIH3T3 cells left unstimulated (1) and stimulated with PDGF (2-5) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4°C, then were incubated with 0.50
µg/mL ab5443 antibody for two hours at room temperature in a 1% BSA-TBST buffer, following prior incubation with: no peptide (1, 2), the non-phosphopeptide corresponding to the immunogen (3), a generic phosphotyrosine containing peptide (4), or, the phosphopeptide immunogen (5). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and bands were detected using the Tropix WesternStar method. The data show that only the peptide corresponding to ab5443 blocks the antibody signal, thereby demonstrating the specificity of the antibody. Peptide Competition: Extracts prepared from NIH3T3 cells left unstimulated (1) and stimulated with PDGF
ab5443 has been referenced in 4 publications.
- Mahadik K et al. c-Abl-TWIST1 Epigenetically Dysregulate Inflammatory Responses during Mycobacterial Infection by Co-Regulating Bone Morphogenesis Protein and miR27a. Front Immunol 9:85 (2018). PubMed: 29449840
- Ip CKM et al. Neomorphic PDGFRA extracellular domain driver mutations are resistant to PDGFRA targeted therapies. Nat Commun 9:4583 (2018). PubMed: 30389923
- Csak T et al. MicroRNA-155 Deficiency Attenuates Liver Steatosis and Fibrosis without Reducing Inflammation in a Mouse Model of Steatohepatitis. PLoS One 10:e0129251 (2015). WB ; Mouse . PubMed: 26042593
- Paugh BS et al. Novel oncogenic PDGFRA mutations in pediatric high-grade gliomas. Cancer Res 73:6219-29 (2013). Human . PubMed: 23970477