Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6.0
Permeabilization
Yes - 0.05% Tween 20
Specification
Kidney
Blocking step
Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Sea Block
Dilution
1/100
Secondary antibody
Dilution
1/300
Name
Non-Abcam antibody was used: Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
Additional data
Additional Notes
Pseudo colored Green immunofluorescent staining of frozen mouse kidney with purified ab32570 Anti-PDGFR beta at a working dilution of 1 in 100. The nuclei are Blue after staining with Hoechst and a light Red counter stain was created with a methyl green staining protocol. The antigen retrieval was performed with the NxGen decloaking chamber at pH6.0 . Alexa Fluor 647 secondary offers better resolution and lacks autofluorescence.
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Submitted Mar 11 2020