Anti-PDGFR alpha antibody (ab124392)
- Datasheet
- References (2)
- Protocols
Overview
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Product nameAnti-PDGFR alpha antibody
See all PDGFR alpha primary antibodies -
DescriptionRabbit polyclonal to PDGFR alpha
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IP, IHC-Pmore details
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Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Chicken, Cow, Dog, Pig, Xenopus laevis
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Immunogen
Synthetic peptide corresponding to Human PDGFR alpha aa 1035-1053 conjugated to keyhole limpet haemocyanin.
Sequence:cGKRNRHSSQTSEESAIETG
Database link: P16234 -
Positive control
- IHC-P: Human brain cerebellum tissue.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze.
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Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 99% Tris glycine, 0.88% Sodium chloride -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab124392 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/250 - 1/500. Detects a band of approximately 150,170 kDa (predicted molecular weight: 122 kDa). | |
| IP | Use at an assay dependent concentration. 5-10 µl immunoprecipitates PDGF Receptor alpha from 500 µg of NIH/3T3 RIPA lysate. | |
| IHC-P | 1/100. |
Target
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FunctionReceptor that binds both PDGFA and PDGFB and has a tyrosine-protein kinase activity.
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Tissue specificityExpressed in primary and metastatic colon tumors and in normal colon tissue. Tumors may express a different isoform to that found in normal tissue.
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Involvement in diseaseNote=A chromosomal aberration involving PDGFRA is found in some cases of hypereosinophilic syndrome. Interstitial chromosomal deletion del(4)(q12q12) causes the fusion of FIP1L1 and PDGFRA (FIP1L1-PDGFRA).
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Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain. -
Cellular localizationMembrane.
- Information by UniProt
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Database links
- Entrez Gene: 395509 Chicken
- Entrez Gene: 442860 Dog
- Entrez Gene: 5156 Human
- Entrez Gene: 18595 Mouse
- Entrez Gene: 100627123 Pig
- Entrez Gene: 25267 Rat
- Omim: 173490 Human
- SwissProt: P16234 Human
see all -
Alternative names
- Alpha-type platelet-derived growth factor receptor antibody
- CD140 antigen-like family member A antibody
- CD140a antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGFR alpha antibody (ab124392)ab124392, at 1/100 dilution, staining PDGFR alpha in formalin-fixed, paraffin-embedded human brain cerebellum tissue by Immunohistochemistry.
Datasheets and documents
References
This product has been referenced in:
- Stammnitz MR et al. The Origins and Vulnerabilities of Two Transmissible Cancers in Tasmanian Devils. Cancer Cell 33:607-619.e15 (2018). IHC-P . Read more (PubMed: 29634948) »
- Steger CM et al. Gene therapy with antisense oligonucleotides silencing c-myc reduces neointima formation and vessel wall thickness in a mouse model of vein graft disease. Exp Mol Pathol 105:1-9 (2018). IHC-P ; Mouse . Read more (PubMed: 29775572) »
