Anti-PDGFR alpha antibody (ab124392)

Reviews (3) Submit a question References (2)

Overview

  • Product name

    Anti-PDGFR alpha antibody
    See all PDGFR alpha primary antibodies

  • Description

    Rabbit polyclonal to PDGFR alpha

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Chicken, Cow, Dog, Pig, Xenopus laevis

  • Immunogen

    Synthetic peptide corresponding to Human PDGFR alpha aa 1035-1053 conjugated to keyhole limpet haemocyanin.
    Sequence:

    cGKRNRHSSQTSEESAIETG


    Database link: P16234

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • IHC-P: Human brain cerebellum tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab124392 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/250 - 1/500. Detects a band of approximately 150,170 kDa (predicted molecular weight: 122 kDa).
IP Use at an assay dependent concentration. 5-10 µl immunoprecipitates PDGF Receptor alpha from 500 µg of NIH/3T3 RIPA lysate.
IHC-P 1/100.

Target

  • Function

    Receptor that binds both PDGFA and PDGFB and has a tyrosine-protein kinase activity.

  • Tissue specificity

    Expressed in primary and metastatic colon tumors and in normal colon tissue. Tumors may express a different isoform to that found in normal tissue.

  • Involvement in disease

    Note=A chromosomal aberration involving PDGFRA is found in some cases of hypereosinophilic syndrome. Interstitial chromosomal deletion del(4)(q12q12) causes the fusion of FIP1L1 and PDGFRA (FIP1L1-PDGFRA).

  • Sequence similarities

    Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Alpha-type platelet-derived growth factor receptor antibody
    • CD140 antigen-like family member A antibody
    • CD140a antibody
    • CD140a antigen antibody
    • MGC74795 antibody
    • PDGF alpha chain antibody
    • PDGF Receptor alpha antibody
    • PDGF-R-alpha antibody
    • PDGFR 2 antibody
    • PDGFR alpha antibody
    • PDGFR2 antibody
    • PDGFRA antibody
    • PDGFRA/BCR fusion antibody
    • PGFRA_HUMAN antibody
    • Platelet derived growth factor receptor 2 antibody
    • Platelet derived growth factor receptor alpha antibody
    • Platelet derived growth factor receptor alpha polypeptide antibody
    • Platelet derived growth factor receptor antibody
    • Rearranged in hypereosinophilia platelet derived growth factor receptor alpha fusion protein antibody
    • RHEPDGFRA antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGFR alpha antibody (ab124392)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGFR alpha antibody (ab124392)

    ab124392, at 1/100 dilution, staining PDGFR alpha in formalin-fixed, paraffin-embedded human brain cerebellum tissue by Immunohistochemistry.

References

This product has been referenced in:

  • Stammnitz MR  et al. The Origins and Vulnerabilities of Two Transmissible Cancers in Tasmanian Devils. Cancer Cell 33:607-619.e15 (2018). IHC-P . Read more (PubMed: 29634948) »
  • Steger CM  et al. Gene therapy with antisense oligonucleotides silencing c-myc reduces neointima formation and vessel wall thickness in a mouse model of vein graft disease. Exp Mol Pathol 105:1-9 (2018). IHC-P ; Mouse . Read more (PubMed: 29775572) »
See all 2 Publications for this product

Publishing research using ab124392? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-3 of 3 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PDGFR alpha antibody

 Good
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Sarcophilus harrisii Tissue sections (Spleen)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer
Permeabilization
No
Specification
Spleen
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Formaldehyde
Read More

Dr. Rachel Owen

Verified customer

Submitted Jan 16 2018

Immunohistochemistry (Frozen sections) abreview for Anti-PDGFR alpha antibody

 Poor
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain)
Permeabilization
No
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More

Juliana Schneider

Verified customer

Submitted Feb 20 2017

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PDGFR alpha antibody

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (Heart)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate pH6.0
Permeabilization
No
Specification
Heart
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Formalin-fixed paraffin-embedded sections
Read More

Abcam user community

Verified customer

Submitted Aug 04 2016

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