Anti-PDGFR alpha antibody (ab96806)

Overview

  • Product name
  • Description
    Rabbit polyclonal to PDGFR alpha
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Dog
  • Immunogen

    Synthetic peptide corresponding to Human PDGFR alpha aa 750-850 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab107908)

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: Caco 2; SW480; MCF7; Jurkat; HepG2; NIH 3T3; PC12.

Properties

Applications

Our Abpromise guarantee covers the use of ab96806 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 mg/ml. Detects a band of approximately 123 kDa (predicted molecular weight: 123 kDa).
IHC-FoFr Use at an assay dependent concentration.

Target

  • Function
    Receptor that binds both PDGFA and PDGFB and has a tyrosine-protein kinase activity.
  • Tissue specificity
    Expressed in primary and metastatic colon tumors and in normal colon tissue. Tumors may express a different isoform to that found in normal tissue.
  • Involvement in disease
    Note=A chromosomal aberration involving PDGFRA is found in some cases of hypereosinophilic syndrome. Interstitial chromosomal deletion del(4)(q12q12) causes the fusion of FIP1L1 and PDGFRA (FIP1L1-PDGFRA).
  • Sequence similarities
    Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alpha-type platelet-derived growth factor receptor antibody
    • CD140 antigen-like family member A antibody
    • CD140a antibody
    • CD140a antigen antibody
    • MGC74795 antibody
    • PDGF alpha chain antibody
    • PDGF Receptor alpha antibody
    • PDGF-R-alpha antibody
    • PDGFR 2 antibody
    • PDGFR alpha antibody
    • PDGFR2 antibody
    • PDGFRA antibody
    • PDGFRA/BCR fusion antibody
    • PGFRA_HUMAN antibody
    • Platelet derived growth factor receptor 2 antibody
    • Platelet derived growth factor receptor alpha antibody
    • Platelet derived growth factor receptor alpha polypeptide antibody
    • Platelet derived growth factor receptor antibody
    • Rearranged in hypereosinophilia platelet derived growth factor receptor alpha fusion protein antibody
    • RHEPDGFRA antibody
    see all

Images

  • All lanes : Anti-PDGFR alpha antibody (ab96806) at 1 µg/ml

    Lane 1 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
    Lane 2 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate
    Lane 3 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 123 kDa
    Observed band size: 123 kDa
    Additional bands at: 32 kDa, 40 kDa, 70 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 8 minutes


    The PDGFR Alpha protein contains a number of potential glycosylation sites (SwissProt). This might explain the migration pattern observed.

  • Immunohistochemistry (PFA perfusion fixed frozen sections) analysis of rat brain tissue sections labeling PDGFR alpha with ab96806 at 1/100 dilution. The sections used came from animals perfused fixed with Paraformaldehyde 4% with 15% of a solution of saturated picric acid, in phosphate buffer 0.1M. Following postfixation in the same fixative overnight, the spinal cord were cryoprotected in sucrose 30% overnight. Spinal cords were then cut using a cryostat and the immunostainings were performed using the ‘free floating’ technique. Tissue was incubated with Anti-PDGF Receptor alpha antibody (ab96806) for 18 hours at 20°C in PBST. A Goat anti-rabbit Alexa Fluor 488 secondary antibody was used at 1/1000 dilution.

     

    See Abreview

  • All lanes : Anti-PDGFR alpha antibody (ab96806) at 1 µg/ml

    Lane 1 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 2 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 123 kDa
    Observed band size: 123 kDa
    Additional bands at: 35 kDa, 75 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 8 minutes

References

ab96806 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Small intestine)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Small intestine
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

Submitted Jul 13 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Embryonic lung)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Embryonic lung
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

Submitted Jul 13 2016

Application
Western blot
Sample
Mouse Cell lysate - whole cell (OliNeu)
Gel Running Conditions
Reduced Denaturing (7,5% gel)
Loading amount
50 µg
Specification
OliNeu
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Abcam user community

Verified customer

Submitted Oct 28 2014

Thank you for your reply.

No problem to wait until October to decide on an appropriate resolution.
My colleagues from the lab are currently investigating the issue with ab96806.

Have a nice holiday.

Thank you for contacting us.

I am sorry to hear this antibody, like ab61219, is not providing clear and satisfactory results.

According to SwissProt (http://www.uniprot.org/uniprot/P16234), PDGF Receptor alpha has 3 isoforms, their si...

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Thank you for your email.

I have issued a free of charge replacement with the alternative antibody ab96806 with the order number xx. The antibody should arrive tomorrow at your place. To check the status of the order please contact our Custo...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Brain)
Specification
Brain
Fixative
Paraformaldehyde
Permeabilization
No
Username

Dr. Sophie Pezet

Verified customer

Submitted Feb 15 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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