Overview

  • Product name

    Anti-PDGFR alpha antibody [APA5]
    See all PDGFR alpha primary antibodies
  • Description

    Rat monoclonal [APA5] to PDGFR alpha
  • Host species

    Rat
  • Tested applications

    Suitable for: WB, IHC-Fr, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Fusion protein corresponding to Mouse PDGFR alpha (extracellular). Fusion protein containing the extracellular domain of Mouse PDGFR alpha and the Fc portion of Human IgG1 purified from the culture supernatant of transfected COS1 cells

  • Positive control

    • NIH3T3 cells

Properties

Applications

Our Abpromise guarantee covers the use of ab90967 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-Fr
Flow Cyt
  • Application notes
    Flow Cyt: Use 1µg for 106 cells in a 100 µl total staining volume.
    IHC-Fr: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 123 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Receptor that binds both PDGFA and PDGFB and has a tyrosine-protein kinase activity.
    • Tissue specificity

      Expressed in primary and metastatic colon tumors and in normal colon tissue. Tumors may express a different isoform to that found in normal tissue.
    • Involvement in disease

      Note=A chromosomal aberration involving PDGFRA is found in some cases of hypereosinophilic syndrome. Interstitial chromosomal deletion del(4)(q12q12) causes the fusion of FIP1L1 and PDGFRA (FIP1L1-PDGFRA).
    • Sequence similarities

      Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
      Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 1 protein kinase domain.
    • Cellular localization

      Membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • Alpha-type platelet-derived growth factor receptor antibody
      • CD140 antigen-like family member A antibody
      • CD140a antibody
      • CD140a antigen antibody
      • MGC74795 antibody
      • PDGF alpha chain antibody
      • PDGF Receptor alpha antibody
      • PDGF-R-alpha antibody
      • PDGFR 2 antibody
      • PDGFR alpha antibody
      • PDGFR2 antibody
      • PDGFRA antibody
      • PDGFRA/BCR fusion antibody
      • PGFRA_HUMAN antibody
      • Platelet derived growth factor receptor 2 antibody
      • Platelet derived growth factor receptor alpha antibody
      • Platelet derived growth factor receptor alpha polypeptide antibody
      • Platelet derived growth factor receptor antibody
      • Rearranged in hypereosinophilia platelet derived growth factor receptor alpha fusion protein antibody
      • RHEPDGFRA antibody
      see all

    Images

    • Staining of NIH/3T3 cells with 0.5 µg Rat IgG2a control antibody (open histogram) or 0.5 µg of ab90967 (colored histogram) followed by Biotin Anti-Rat IgG secondary antibody. Total viable cells were analysed.

    References

    This product has been referenced in:

    • Jang M  et al. Oligodendrocytes regulate presynaptic properties and neurotransmission through BDNF signaling in the mouse brainstem. Elife 8:N/A (2019). Read more (PubMed: 30998186) »
    • Ntokou A  et al. Characterization of the platelet-derived growth factor receptor-a-positive cell lineage during murine late lung development. Am J Physiol Lung Cell Mol Physiol 309:L942-58 (2015). Read more (PubMed: 26320158) »
    See all 6 Publications for this product

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Question
    Answer

    Thank you for contacting Abcam regarding ab90967.


    We have not tested this antibody directly in-house for cross reactivity against CD140b.The clone is APA5 and according to the development paper for this clone, this antibody is specific for Cd140a and does not bind CD140b.


    http://jhc.sagepub.com/content/45/6/883.full.pdf+html

    I hope this information is helpful. Please do not hesitate to contact me if you have any additional questions.

    Read More
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (brain)
    Permeabilization
    Yes - 0.3%Triton x-100
    Specification
    brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Fixative
    Paraformaldehyde

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    Verified customer

    Submitted Nov 14 2018

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