Anti-PDGFR alpha antibody [APA5] - Low endotoxin, Azide free (ab171230)

Overview

  • Product name
    Anti-PDGFR alpha antibody [APA5] - Low endotoxin, Azide free
    See all PDGFR alpha primary antibodies
  • Description
    Rat monoclonal [APA5] to PDGFR alpha - Low endotoxin, Azide free
  • Host species
    Rat
  • Tested applications
    Suitable for: Flow Cyt, Functional Studies, WB, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Fusion protein corresponding to Mouse PDGFR alpha (extracellular). Fusion protein containing the extracellular domain of Mouse PDGF Receptor alpha and the Fc portion of Human IgG1 purified from the culture supernatant of transfected COS1 cells

  • Positive control
    • Mouse NIH 3T3 cell line
  • General notes

    Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by the LAL assay.

Properties

Applications

Our Abpromise guarantee covers the use of ab171230 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 105-8 cells.

(Final test volume = 100 µL)

ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Functional Studies Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 123 kDa.
IHC-Fr Use at an assay dependent concentration.

Target

  • Function
    Receptor that binds both PDGFA and PDGFB and has a tyrosine-protein kinase activity.
  • Tissue specificity
    Expressed in primary and metastatic colon tumors and in normal colon tissue. Tumors may express a different isoform to that found in normal tissue.
  • Involvement in disease
    Note=A chromosomal aberration involving PDGFRA is found in some cases of hypereosinophilic syndrome. Interstitial chromosomal deletion del(4)(q12q12) causes the fusion of FIP1L1 and PDGFRA (FIP1L1-PDGFRA).
  • Sequence similarities
    Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alpha-type platelet-derived growth factor receptor antibody
    • CD140 antigen-like family member A antibody
    • CD140a antibody
    • CD140a antigen antibody
    • MGC74795 antibody
    • PDGF alpha chain antibody
    • PDGF Receptor alpha antibody
    • PDGF-R-alpha antibody
    • PDGFR 2 antibody
    • PDGFR alpha antibody
    • PDGFR2 antibody
    • PDGFRA antibody
    • PDGFRA/BCR fusion antibody
    • PGFRA_HUMAN antibody
    • Platelet derived growth factor receptor 2 antibody
    • Platelet derived growth factor receptor alpha antibody
    • Platelet derived growth factor receptor alpha polypeptide antibody
    • Platelet derived growth factor receptor antibody
    • Rearranged in hypereosinophilia platelet derived growth factor receptor alpha fusion protein antibody
    • RHEPDGFRA antibody
    see all

Images

  • Flow cytometry analysis of NIH 3T3 cells using ab171230 at 0.5 µg (blue histogram) or purified Rat IgG2a kappa Isotype Control (open histogram) at 0.5 µg; Followed by anti-Rat IgG Biotin and Streptavidin PE. Total viable cells were used for analysis.

References

ab171230 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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