Recombinant
RabMAb

Recombinant Anti-PDGFR alpha antibody [EPR22059-270] - BSA and Azide free (ab234965)

Overview

  • Product name
    Anti-PDGFR alpha antibody [EPR22059-270] - BSA and Azide free
    See all PDGFR alpha primary antibodies
  • Description
    Rabbit monoclonal [EPR22059-270] to PDGFR alpha - BSA and Azide free
  • Host species
    Rabbit
  • Specificity
    IHC is recommended for mouse and rat only.
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment aa 1-550. The exact sequence is proprietary.
    Database link: P16234

  • Positive control
    • IHC-P: Mouse E14.5 lung tissue.
  • General notes

    The formulation and the concentration of this product is compatible for metal-conjugation for mass cytometry (CyTOF®).

    ab234965 is a PBS-only buffer format of ab203491. Please refer to ab203491 for recommended dilutions, protocols, and image data.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab234965 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 150, 180 kDa (predicted molecular weight: 122 kDa).
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC is recommended for mouse and rat only.

ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

Target

  • Function
    Receptor that binds both PDGFA and PDGFB and has a tyrosine-protein kinase activity.
  • Tissue specificity
    Expressed in primary and metastatic colon tumors and in normal colon tissue. Tumors may express a different isoform to that found in normal tissue.
  • Involvement in disease
    Note=A chromosomal aberration involving PDGFRA is found in some cases of hypereosinophilic syndrome. Interstitial chromosomal deletion del(4)(q12q12) causes the fusion of FIP1L1 and PDGFRA (FIP1L1-PDGFRA).
  • Sequence similarities
    Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alpha-type platelet-derived growth factor receptor antibody
    • CD140 antigen-like family member A antibody
    • CD140a antibody
    • CD140a antigen antibody
    • MGC74795 antibody
    • PDGF alpha chain antibody
    • PDGF Receptor alpha antibody
    • PDGF-R-alpha antibody
    • PDGFR 2 antibody
    • PDGFR alpha antibody
    • PDGFR2 antibody
    • PDGFRA antibody
    • PDGFRA/BCR fusion antibody
    • PGFRA_HUMAN antibody
    • Platelet derived growth factor receptor 2 antibody
    • Platelet derived growth factor receptor alpha antibody
    • Platelet derived growth factor receptor alpha polypeptide antibody
    • Platelet derived growth factor receptor antibody
    • Rearranged in hypereosinophilia platelet derived growth factor receptor alpha fusion protein antibody
    • RHEPDGFRA antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded rat E14.5 intervertebral disc tissue labeling PDGFR alpha with ab203491 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining in mesenchymal cells of rat E14.5 intervertebral disc (PMID: 9199674). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab203491).

  • PDGFR alpha was immunoprecipitated from 0.35 mg of A-204 (human muscle rhabdomyosarcoma cell line) whole cell lysate with ab203491 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab203491 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

    Lane 1: A-204 whole cell lysate 10 µg (Input). 

    Lane 2: ab203491 IP in A-204 whole cell lysate. 

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab203491 in A-204 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 50 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab203491).

  • Flow cytometric analysis of A-172 (human brain glioblastoma cell line, left) and A-204 (human muscle rhabdomyosarcoma cell line, right) cell lines labeling PDGFR alpha with ab203491 at 1/50 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

    Gated on viable cells.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab203491).

  • Flow cytometric analysis of NIH/3T3 (mouse embyro fibroblast cell line) cell line labeling PDGFR alpha with ab203491 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

    Gated on viable cells.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab203491).

  • Immunohistochemical analysis of paraffin-embedded mouse uterus tissue labeling PDGFR alpha with ab203491 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on interstitial cells of mouse uterus (PMID: 25788664). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab203491).

  • Immunohistochemical analysis of paraffin-embedded mouse E14.5 intervertebral disc tissue labeling PDGFR alpha with ab203491 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining in mesenchymal cells of mouse E14.5 intervertebral disc (PMID: 9199674). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab203491).

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A-204 (human muscle rhabdomyosarcoma cell line) cells and A-172 (human brain glioblastoma cell line) labeling PDGFR alpha with ab203491 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Confocal image showing membranous and cytoplasmic staining in A-204 cell line.

    Negative control: A-172 (PMID:8425771.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab203491).

  • Immunohistochemical analysis of paraffin-embedded mouse E14.5 lung tissue labeling PDGFR alpha with ab203491 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining in the mesenchyme of mouse E14.5 lung (PMID: 8681381). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab203491).

References

ab234965 has not yet been referenced specifically in any publications.

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