Recombinant
RabMAb

Recombinant Anti-PDGFR alpha (phospho Y720) antibody [EP2478] (ab134068)

Overview

  • Product name

    Anti-PDGFR alpha (phospho Y720) antibody [EP2478]
    See all PDGFR alpha primary antibodies
  • Description

    Rabbit monoclonal [EP2478] to PDGFR alpha (phospho Y720)
  • Host species

    Rabbit
  • Specificity

    ab134068 only detects PDGFR alpha phosphorylated at Tyrosine 720.

  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    corresponding to Human PDGFR alpha.

  • Positive control

    • NIH 3T3 cell lysates, treated with Pervanadate.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab134068 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 190 kDa (predicted molecular weight: 122 kDa).
Flow Cyt 1/200 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC,IHC-P or IP.
  • Target

    • Function

      Receptor that binds both PDGFA and PDGFB and has a tyrosine-protein kinase activity.
    • Tissue specificity

      Expressed in primary and metastatic colon tumors and in normal colon tissue. Tumors may express a different isoform to that found in normal tissue.
    • Involvement in disease

      Note=A chromosomal aberration involving PDGFRA is found in some cases of hypereosinophilic syndrome. Interstitial chromosomal deletion del(4)(q12q12) causes the fusion of FIP1L1 and PDGFRA (FIP1L1-PDGFRA).
    • Sequence similarities

      Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
      Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 1 protein kinase domain.
    • Cellular localization

      Membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • Alpha-type platelet-derived growth factor receptor antibody
      • CD140 antigen-like family member A antibody
      • CD140a antibody
      • CD140a antigen antibody
      • MGC74795 antibody
      • PDGF alpha chain antibody
      • PDGF Receptor alpha antibody
      • PDGF-R-alpha antibody
      • PDGFR 2 antibody
      • PDGFR alpha antibody
      • PDGFR2 antibody
      • PDGFRA antibody
      • PDGFRA/BCR fusion antibody
      • PGFRA_HUMAN antibody
      • Platelet derived growth factor receptor 2 antibody
      • Platelet derived growth factor receptor alpha antibody
      • Platelet derived growth factor receptor alpha polypeptide antibody
      • Platelet derived growth factor receptor antibody
      • Rearranged in hypereosinophilia platelet derived growth factor receptor alpha fusion protein antibody
      • RHEPDGFRA antibody
      see all

    Images

    • All lanes : Anti-PDGFR alpha (phospho Y720) antibody [EP2478] (ab134068) at 1/1000 dilution

      Lane 1 : NIH 3T3 cell lysate, untreated
      Lane 2 : NIH 3T3 cell lysate, treated with Pervanadate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 122 kDa
      Observed band size: 190 kDa
      why is the actual band size different from the predicted?

    • Flow cytometry analysis of NIH/3T3 cells treated with 1mM Pervanadate for 30mins cells labeling PDGFR alpha (phospho Y720) with unpurified ab134068 at 1/200 dilution (0.0445µg) (red). Cells were fixed with 4% paraformaldehyde . Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730 /Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

    • Flow Cytometry analysis of permeabilized NIH/3T3 cells labelling PDGFR alpha (phospho Y720) with ab134068. Green - untreated, Red - pervanadate-treated, Blue - preincubated with phospho-PDGF Receptor alpha (phospho Y720) peptide, Orange - preincubated with non-phospho-PDGR Receptor alpha peptide.

    References

    This product has been referenced in:

    • Sanada TJ  et al. Characterization of pulmonary intimal sarcoma cells isolated from a surgical specimen: In vitro and in vivo study. PLoS One 14:e0214654 (2019). Read more (PubMed: 30925179) »
    See 1 Publication for this product

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Answer

    Thank you for your reply. I apologize for the confusion, I thought you were saying that 45 minutes was the exposure time for the film. I am not an expert in PDGF do I do not know what prolonged stimulation with PDGF would do. I would recommend using a higher percent gel, since your expected band should be pretty close to the top, but I think that bright band you are seeing is not your true target band but rather an artifact where maybe some of the sample has aggregated. I would also block for 2 hours at room temperature and then incubate overnight at 4 degrees with the primary, rather than the other way around. Please let me know if these suggestions do not improve your result.

    Read More

    Answer

    Thank you for your message.

    As agreed, I have issued a free of charge replacement with one unit of ab134068; the order number is *****.

    To check the status of the order please contact our Customer Service team and reference this number.

    Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

    I wish you the best of luck with your research.

    Read More

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