Key features and details
- Rabbit polyclonal to PDGFR alpha (phospho Y849)
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-PDGFR alpha (phospho Y849) antibody
See all PDGFR alpha primary antibodies
DescriptionRabbit polyclonal to PDGFR alpha (phospho Y849)
Specificityab79318 detects endogenous levels of PDGFR alpha only when phosphorylated at tyrosine 849.
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
- Purchase matching WB positive control:Recombinant human PDGFR alpha protein
- 293 cell extracts.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThe antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
Our Abpromise guarantee covers the use of ab79318 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 133 kDa (predicted molecular weight: 123 kDa).|
FunctionReceptor that binds both PDGFA and PDGFB and has a tyrosine-protein kinase activity.
Tissue specificityExpressed in primary and metastatic colon tumors and in normal colon tissue. Tumors may express a different isoform to that found in normal tissue.
Involvement in diseaseNote=A chromosomal aberration involving PDGFRA is found in some cases of hypereosinophilic syndrome. Interstitial chromosomal deletion del(4)(q12q12) causes the fusion of FIP1L1 and PDGFRA (FIP1L1-PDGFRA).
Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain.
- Information by UniProt
- Alpha-type platelet-derived growth factor receptor antibody
- CD140 antigen-like family member A antibody
- CD140a antibody
All lanes : Anti-PDGFR alpha (phospho Y849) antibody (ab79318) at 1/500 dilution
Lane 1 : 293 cells extract
Lane 2 : 293 cells extract with Immunizing phosphopeptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 123 kDa
Observed band size: 133 kDa why is the actual band size different from the predicted?
ab79318 has been referenced in 1 publication.
- Tsoli M et al. Dual targeting of mitochondrial function and mTOR pathway as a therapeutic strategy for diffuse intrinsic pontine glioma. Oncotarget 9:7541-7556 (2018). PubMed: 29484131