Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PDGF Receptor alpha (phospho Y849) antibody

Good
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (brain)
Specification
brain
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citric acid pH 6.0
Permeabilization
No
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 0.5% · Temperature: RT°C

Other product details

Dilution
1/50
Incubation time
1 hour(s) and 0 minute(s) · Diluent: 0.5 % BSA/PBS

Secondary antibody

Name
Non-Abcam antibody was used: Dako REAL™ HRP/DAB+, Rabbit/Mouse
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Additional data

Additional Notes
Nuclei staining with Haematoxillin (blue) and PDGF receptor alpha staining with DAB (brown). We found membranouse staining of glia cells within the white matter of the mouse brain.

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Submitted Nov 16 2010

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