Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PDGF Receptor alpha (phospho Y849) antibody

Good
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (glioblastoma multiforme brain section)
Specification
glioblastoma multiforme brain section
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citric acid pH 6.0
Permeabilization
No
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 0.5% · Temperature: RT°C

Other product details

Dilution
1/50
Incubation time
1 hour(s) and 0 minute(s) · Diluent: 0.5 % BSA/PBS

Secondary antibody

Name
Non-Abcam antibody was used: Dako REAL™ HRP/DAB+, Rabbit/Mouse
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Additional data

Additional Notes
Nuclei staining with haematoxillin (blue) and PDGR Receptor alpha staining with DAB (brown). We found a distinct staining of a few neoplastic glia cells in tumor tissue.

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Submitted Nov 16 2010

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