Immunocytochemistry/ Immunofluorescence abreview for Anti-PDGF Receptor alpha (phospho Y849) antibody

Average
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (human glioblastoma cell line)
Specification
human glioblastoma cell line
Fixative
Formaldehyde
Permeabilization
Yes - Tritonx100 0.1% in PBS
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 0.5% · Temperature: RT°C

Other product details

Dilution
1/50
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 0.5 % BSA/PBS

Secondary antibody

Name
Non-Abcam antibody was used: F0382 Anti Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy3 ®
Dilution
1/400

Additional data

Additional Notes
We found a staining in the cell membrane but also in the nucleus. Nucleii are counterstained with Hoechst. Secondary AB conjugated with Cy3 (red)

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Submitted Apr 29 2011

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